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PMID:15661000

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Citation

Kearns, DB, Chu, F, Branda, SS, Kolter, R and Losick, R (2005) A master regulator for biofilm formation by Bacillus subtilis. Mol. Microbiol. 55:739-49

Abstract

Wild strains of Bacillus subtilis are capable of forming architecturally complex communities of cells known as biofilms. Critical to biofilm formation is the eps operon, which is believed to be responsible for the biosynthesis of an exopolysaccharide that binds chains of cells together in bundles. We report that transcription of eps is under the negative regulation of SinR, a repressor that was found to bind to multiple sites in the regulatory region of the operon. Mutations in sinR bypassed the requirement in biofilm formation of two genes of unknown function, ylbF and ymcA, and sinI, which is known to encode an antagonist of SinR. We propose that these genes are members of a pathway that is responsible for counteracting SinR-mediated repression. We further propose that SinR is a master regulator that governs the transition between a planktonic state in which the bacteria swim as single cells in liquid or swarm in small groups over surfaces, and a sessile state in which the bacteria adhere to each other to form bundled chains and assemble into multicellular communities.

Links

PubMed Online version:10.1111/j.1365-2958.2004.04440.x

Keywords

Amino Acid Sequence; Bacillus subtilis/genetics; Bacillus subtilis/growth & development; Bacillus subtilis/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Biofilms/growth & development; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mutation; Operon; Polysaccharides, Bacterial/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BACSU:SINR

GO:0010629: negative regulation of gene expression

ECO:0000315:

P

Table 1

complete
CACAO 2856

BACSU:SINR

involved_in

GO:0010629: negative regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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