GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15654100

From GONUTS
Jump to: navigation, search
Citation

Holway, AH, Hung, C and Michael, WM (2005) Systematic, RNA-interference-mediated identification of mus-101 modifier genes in Caenorhabditis elegans. Genetics 169:1451-60

Abstract

The Mus101 family of chromosomal proteins, identified initially in Drosophila, is widely conserved and has been shown to function in a variety of DNA metabolic processes. Such functions include DNA replication, DNA damage repair, postreplication repair, damage checkpoint activation, chromosome stability, and chromosome condensation. Despite its conservation and widespread involvement in chromosome biogenesis, very little is known about how Mus101 is regulated and what other proteins are required for Mus101 to exert its functions. To learn more about Mus101, we have initiated an analysis of the protein in C. elegans. Here, we show that C. elegans mus-101 is an essential gene, that it is required for DNA replication, and that it also plays an important role in the DNA damage response. Furthermore, we use RNA interference (RNAi)-mediated reverse genetics to screen for genes that modify a mus-101 partial loss-of-function RNAi phenotype. Using a systematic approach toward modifier gene discovery, we have found five chromosome I genes that modify the mus-101 RNAi phenotype, and we go on to show that one of them encodes an E3 SUMO ligase that promotes SUMO modification of MUS-101 in vitro. These results expand our understanding of MUS-101 regulation and show that genetic interactions can be uncovered using screening strategies that rely solely on RNAi.

Links

PubMed PMC1449550 Online version:10.1534/genetics.104.036137

Keywords

Animals; Caenorhabditis elegans/drug effects; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Chromosomal Proteins, Non-Histone/genetics; Chromosome Mapping; Embryo, Nonmammalian/physiology; Methyl Methanesulfonate/pharmacology; Mutagens/pharmacology; RNA Interference; Tubulin/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:PLIN1

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00009507

P

Seeded From UniProt

complete

CAEEL:HRPK1

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00009507

P

Seeded From UniProt

complete

CAEEL:HRPK1

involved_in

GO:0006974: cellular response to DNA damage stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00007024

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00002324

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00017816

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00001574

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0006974: cellular response to DNA damage stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0006261: DNA-dependent DNA replication

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q20129

involved_in

GO:0036093: germ cell proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:GEI17

involved_in

GO:0016925: protein sumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:GEI17

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00009507

P

Seeded From UniProt

complete

CAEEL:GEI17

involved_in

GO:0006974: cellular response to DNA damage stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:GEI17

enables

GO:0061665: SUMO ligase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(WB:WBGene00009507)

Seeded From UniProt

complete

CAEEL:MED7

involved_in

GO:0000003: reproduction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:MED7

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00009507

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.