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PMID:15645444

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Citation

Park, YK, Franklin, JL, Settle, SH, Levy, SE, Chung, E, Jeyakumar, LH, Shyr, Y, Washington, MK, Whitehead, RH, Aronow, BJ and Coffey, RJ (2005) Gene expression profile analysis of mouse colon embryonic development. Genesis 41:1-12

Abstract

During late embryogenesis, the mouse colon develops from a pseudostratified, undifferentiated endoderm to a single-layered columnar epithelium with accompanying mesenchymal maturation. To identify regulatory genetic programs underlying these morphological changes, we profiled gene expression of the developing mouse colon by microarray from embryonic day (E)13.5 to E18.5. Unbiased cluster analysis of 13,484 cDNA elements revealed two distinct groups of genes whose expression changes reflect the dynamic morphological events of the epithelium and mesenchyme during this period. Additional analyses revealed two subsets of genes whose expression is either upregulated or downregulated over the same developmental period. Of those genes whose expression increases from E13.5 to E18.5 (n = 158), known functions include acquisition and/or maintenance of colonic differentiation. Genes whose transcription is downregulated over this period (n = 49) have demonstrated roles in nuclear organization, transcriptional regulation, and cell proliferation. These results provide the basis for a molecular portrait of colonic development during late embryogenesis and should be a valuable resource for investigators interested in colonic development and neoplasia, as well as comparative organogenesis.

Links

PubMed Online version:10.1002/gene.20088

Keywords

Animals; Animals, Outbred Strains; Cluster Analysis; Colon/embryology; Colon/growth & development; Colon/ultrastructure; DNA, Complementary; Embryonic Development; Epithelium/metabolism; Female; Gene Expression Profiling; Gene Expression Regulation, Developmental; Immunohistochemistry; Mesoderm/metabolism; Mice; Mice, Inbred C57BL; Oligonucleotide Array Sequence Analysis; Pregnancy; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CDK4

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000131)
  • part_of:(EMAPA:18941)|part_of:(CL:0000131)
  • part_of:(EMAPA:17902)|part_of:(CL:0000499)
  • part_of:(EMAPA:17900)

Seeded From UniProt

complete

MOUSE:HES1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000499)
  • part_of:(EMAPA:17900)|part_of:(EMAPA:18939)

Seeded From UniProt

complete

MOUSE:HES1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:18939)

Seeded From UniProt

complete

MOUSE:CTNB1

located_in

GO:0016328: lateral plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:18941)

Seeded From UniProt

complete

MOUSE:CTNB1

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000131)
  • part_of:(EMAPA:17902)

Seeded From UniProt

complete

MOUSE:SOX4

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:18939)|part_of:(EMAPA:16715)

Seeded From UniProt

complete

MOUSE:TFF3

located_in

GO:0030141: secretory granule

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000160)
  • part_of:(EMAPA:18941)

Seeded From UniProt

complete

MOUSE:CTNB1

part_of

GO:0005913: cell-cell adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17902)
  • part_of:(CL:0000131)

Seeded From UniProt

complete

MOUSE:CTNB1

part_of

GO:0016328: lateral plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(EMAPA:18941)

Seeded From UniProt

complete

MOUSE:TFF3

part_of

GO:0030141: secretory granule

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:18941)
  • part_of:(CL:0000160)

Seeded From UniProt

complete

See also

References

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