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PMID:15644333

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Citation

Bezy, O, Elabd, C, Cochet, O, Petersen, RK, Kristiansen, K, Dani, C, Ailhaud, G and Amri, EZ (2005) Delta-interacting protein A, a new inhibitory partner of CCAAT/enhancer-binding protein beta, implicated in adipocyte differentiation. J. Biol. Chem. 280:11432-8

Abstract

CCAAT/enhancer-binding protein beta (C/EBP beta) is expressed early during the adipocyte differentiation program and plays an important role in this process. In an attempt to identify novel proteins that interact with C/EBP beta, we performed a yeast two-hybrid screen with a preadipocyte cDNA library and identified a new co-regulator, delta-interacting protein A (DIPA). DIPA mRNA is expressed during adipocyte differentiation of clonal cell lines. DIPA interacts with C/EBP beta and -delta proteins in intact cells and inhibits their transcriptional activity but not that of C/EBP alpha. Stable overexpression of DIPA in preadipocytes partially inhibits adipocyte differentiation, whereas its gene silencing enhances this process. DIPA and C/EBP beta co-localize in the nucleus, and overexpression of DIPA in preadipocytes results in a partial inhibition of the mitotic clonal expansion which is critical for differentiation. Thus, DIPA is a novel partner of C/EBP beta that down-regulates early events of adipogenesis.

Links

PubMed Online version:10.1074/jbc.M411741200

Keywords

Adaptor Proteins, Signal Transducing; Adipocytes/cytology; Animals; CCAAT-Enhancer-Binding Protein-beta/antagonists & inhibitors; CCAAT-Enhancer-Binding Protein-beta/genetics; Carrier Proteins/physiology; Cell Differentiation; Cells, Cultured; Gene Silencing; Mice; Mitosis; NIH 3T3 Cells; PPAR gamma/metabolism; Promoter Regions, Genetic; Repressor Proteins; Transcription, Genetic; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CEBPB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q6PDY0

F

Seeded From UniProt

complete

MOUSE:CC85B

involved_in

GO:0045599: negative regulation of fat cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CC85B

involved_in

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CC85B

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CC85B

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P28033

F

Seeded From UniProt

complete


See also

References

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