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PMID:15632080

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Citation

Kelleher, C, Kurth, I and Lingner, J (2005) Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro. Mol. Cell. Biol. 25:808-18

Abstract

The telomeric single-strand DNA binding protein protection of telomeres 1 (POT1) protects telomeres from rapid degradation in Schizosaccharomyces pombe and has been implicated in positive and negative telomere length regulation in humans. Human POT1 appears to interact with telomeres both through direct binding to the 3' overhanging G-strand DNA and through interaction with the TRF1 duplex telomere DNA binding complex. The influence of POT1 on telomerase activity has not been studied at the molecular level. We show here that POT1 negatively effects telomerase activity in vitro. We find that the DNA binding activity of POT1 is required for telomerase inhibition. Furthermore, POT1 is incapable of inhibiting telomeric repeat addition to substrate primers that are defective for POT1 binding, suggesting that in vivo, POT1 likely affects substrate access to telomerase.

Links

PubMed PMC543404 Online version:10.1128/MCB.25.2.808-818.2005

Keywords

Animals; Binding Sites; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; HeLa Cells; Humans; Protein Binding; Protein Structure, Tertiary; Telomerase/antagonists & inhibitors; Telomerase/genetics; Telomerase/metabolism; Telomere/metabolism; Telomere-Binding Proteins/genetics; Telomere-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TERT

part_of

GO:0000783: nuclear telomere cap complex

ECO:0000305: curator inference used in manual assertion

GO:0003721

C

Seeded From UniProt

complete

HUMAN:TERT

enables

GO:0003721: telomerase RNA reverse transcriptase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:POTE1

enables

GO:0010521: telomerase inhibitor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:POTE1

enables

GO:0043047: single-stranded telomeric DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:POTE1

involved_in

GO:0051974: negative regulation of telomerase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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