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PMID:15632077
| Citation |
Adams, DJ, van der Weyden, L, Gergely, FV, Arends, MJ, Ng, BL, Tannahill, D, Kanaar, R, Markus, A, Morris, BJ and Bradley, A (2005) BRCTx is a novel, highly conserved RAD18-interacting protein. Mol. Cell. Biol. 25:779-88 |
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| Abstract |
The BRCT domain is a highly conserved module found in many proteins that participate in DNA damage checkpoint regulation, DNA repair, and cell cycle control. Here we describe the cloning, characterization, and targeted mutagenesis of Brctx, a novel gene with a BRCT motif. Brctx was found to be expressed ubiquitously in adult tissues and during development, with the highest levels found in testis. Brctx-deficient mice develop normally, show no pathological abnormalities, and are fertile. BRCTx binds to the C terminus of hRAD18 in yeast two-hybrid and immunoprecipitation assays and colocalizes with this protein in the nucleus. Despite this, Brctx-deficient murine embryonic fibroblasts (MEFs) do not show overt sensitivity to DNA-damaging agents. MEFs from Brctx-deficient embryos grow at a similar rate to wild-type MEF CD4/CD8 expressions, and the cell cycle parameters of thymocytes from wild-type and Brctx knockout animals are indistinguishable. Intriguingly, the BRCT domain of BRCTx is responsible for mediating its localization to the nucleus and centrosome in interphase cells. We conclude that, although highly conserved, Brctx is not essential for the above-mentioned processes and may be redundant. |
| Links |
PubMed PMC543416 Online version:10.1128/MCB.25.2.779-788.2005 |
| Keywords |
Amino Acid Sequence; Animals; Body Weight; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Cycle/physiology; Cells, Cultured; Centrosome/metabolism; DNA Damage; DNA Repair; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Fibroblasts/cytology; Fibroblasts/physiology; Humans; Male; Mice; Mice, Knockout; Molecular Sequence Data; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Organ Size; Protein Binding; Sequence Alignment; T-Lymphocytes/cytology; T-Lymphocytes/physiology; Testis/cytology; Testis/metabolism; Tissue Distribution; Two-Hybrid System Techniques |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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