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PMID:15618410

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Citation

Carrari, F, Coll-Garcia, D, Schauer, N, Lytovchenko, A, Palacios-Rojas, N, Balbo, I, Rosso, M and Fernie, AR (2005) Deficiency of a plastidial adenylate kinase in Arabidopsis results in elevated photosynthetic amino acid biosynthesis and enhanced growth. Plant Physiol. 137:70-82

Abstract

An Arabidopsis (Arabidopsis thaliana) L. Heynh mutant deficient in an isoform of adenylate kinase (ADK; At2g37250) was isolated by reverse genetics. It contains a T-DNA insertion 377 bp downstream of the start point of transcription. The mutant lacks At2g37250 transcripts and has a mild reduction in total cellular ADK activity. Green fluorescent protein-fusion based cellular localization experiments, carried out with the full-length At2g37250, suggested a plastidial localization for this isoform. In keeping with this observation, organelle isolation experiments revealed that the loss in ADK activity was confined to the inner plastid. This plastid stroma ADK gene was found to be expressed tissue constitutively but at much higher levels in illuminated leaves. Phenotypic and biochemical analyses of the mutant revealed that it exhibited higher amino acid biosynthetic activity in the light and was characterized by an enhanced root growth. When the mutant was subjected to either continuous light or continuous dark, growth phenotypes were also observed in the shoots. While the levels of adenylates were not much altered in the leaves, the pattern of change observed in the roots was consistent with the inhibition of an ATP-consuming reaction. Taken together, these data suggest a role for the plastid stromal ADK in the coordination of metabolism and growth, but imply that the exact importance of this isoform is tissue dependent.

Links

PubMed PMC548839 Online version:10.1104/pp.104.056143

Keywords

Adenylate Kinase/genetics; Adenylate Kinase/metabolism; Amino Acids/biosynthesis; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Carbohydrate Metabolism; Circadian Rhythm; DNA, Bacterial; Gene Expression Regulation, Plant; Multigene Family; Mutation; Photosynthesis/physiology; Plant Leaves/metabolism; Plant Roots/metabolism; Plastids/enzymology; Seeds/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:KAD1

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:KAD1

located_in

GO:0009570: chloroplast stroma

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:KAD1

acts_upstream_of_or_within

GO:0008652: cellular amino acid biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:KAD1

enables

GO:0004017: adenylate kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:KAD1

acts_upstream_of_or_within

GO:0048367: shoot system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:KAD1

acts_upstream_of_or_within

GO:0048364: root development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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