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PMID:15613308
| Citation |
Zhu, FX, Chong, JM, Wu, L and Yuan, Y (2005) Virion proteins of Kaposi's sarcoma-associated herpesvirus. J. Virol. 79:800-11 |
|---|---|
| Abstract |
The proteins that compose a herpesvirus virion are thought to contain the functional information required for de novo infection, as well as virion assembly and egress. To investigate functional roles of Kaposi's sarcoma-associated herpesvirus (KSHV) virion proteins in viral productive replication and de novo infection, we attempted to identify virion proteins from purified KSHV by a proteomic approach. Extracellular KSHV virions were purified from phorbol-12-tetradecanoate-13-acetate-induced BCBL-1 cells through double-gradient ultracentrifugation, and their component proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirty prominent protein bands were excised and subjected to high-performance liquid chromatography ion trap mass spectrometric analysis. This study led to the identification of 24 virion-associated proteins. These include five capsid proteins, eight envelope glycoproteins, six tegument proteins, and five proteins whose locations in the virions have not yet been defined. Putative tegument proteins encoded by open reading frame 21 (ORF21), ORF33, and ORF45 were characterized and found to be resistant to protease digestion when purified virions were treated with trypsin, confirming that they are located within the virion particles. The ORF64-encoded large tegument protein was found to be associated with capsid but sensitive to protease treatment, suggesting its unique structure and array in KSHV virions. In addition, cellular beta-actin and class II myosin heavy chain type A were found inside KSHV virions and associated with tegument-capsid structure. Identification of KSHV virion proteins makes it possible to study the functional roles of these virion proteins in KSHV replication and pathogenicity. |
| Links |
PubMed PMC538588 Online version:10.1128/JVI.79.2.800-811.2005 |
| Keywords |
Amino Acid Sequence; Capsid Proteins/analysis; Cell Line, Tumor; Herpesvirus 8, Human/chemistry; Humans; Molecular Sequence Data; Terminology as Topic; Viral Envelope Proteins/analysis; Viral Proteins/analysis; Viral Proteins/chemistry; Viral Proteins/physiology; Virion/chemistry |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF17.5, which codes for a capsid protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF47, which codes for envelope glycoprotein L, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF26, which codes for a triplex capsid protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 3 shows that Cytoplasmic envelopment protein 2, or ORF33, is associated with the pelleted tegument-capsid structure of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). ORF33 is a tegument protein. |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF33, which codes for a cytoplasmic envelopment protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 3 shows that Thymidine kinase, or ORF21 (TK ORF21), is associated with the pelleted tegument-capsid structure of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). ORF21 is a tegument protein. |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF21, which codes for a thymidine kinase, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019033: viral tegument |
ECO:0000314: |
C |
Figure 3 shows that Protein ORF45, or ORF45, is associated with the pelleted tegument-capsid structure of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). ORF45 is a tegument protein. |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF45, which codes for a tegument protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019030: icosahedral viral capsid |
ECO:0000314: |
C |
Figure 3 shows that Large tegument protein deneddylase, or ORF64, is a tegument protein that is associated with the capsid at one end and attached to the envelope at the other end of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). |
complete | ||||
| GO:0019031: viral envelope |
ECO:0000314: |
C |
Figure 3 shows that Large tegument protein deneddylase, or ORF64, is a tegument protein that is associated with the capsid at one end and attached to the envelope at the other end of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). |
complete | ||||
| GO:0019031: viral envelope |
ECO:0000314: |
C |
Table 2 shows that Envelope glycoprotein B, or ORF8 (gB ORF8), is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). |
complete | ||||
| GO:0019031: viral envelope |
ECO:0000314: |
C |
Table 2 shows that Envelope glycoprotein H, or ORF22 (gH ORF22), is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF22, which codes for envelope glycoprotein H, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019031: viral envelope |
ECO:0000314: |
C |
Table 2 shows that Protein ORF28, or ORF28, is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF28, which codes for an envelope protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019031: viral envelope |
ECO:0000314: |
C |
Table 2 shows that Envelope glycoprotein M or ORF39, is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF39, which codes for envelope glycoprotein M, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: protein mass spectrometry evidence used in manual assertion |
C |
Figure 1, lane 4 shows that gene ORF8, which codes for Envelope glycoprotein B, is present in Kaposi’s Sarcoma-associated Herpesvirus virion. |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF64, which codes for a tegument protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF25, which codes for a major capsid protein(MCP), is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF75, which codes for a tegument protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF6, which codes for DNA binding protein(DBP), is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 2 shows that gene ORF63, which codes for an inner tegument protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene product ORF11 is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF7, which codes for a tripartite terminase subunit, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: protein mass spectrometry evidence used in manual assertion |
C |
Figure 1, lane 4 shows that gene ORF27, which codes for an uncategorized protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF62, which codes for a triplex capsid protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF52, which codes for a tegument protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF53, which codes for envelope glycoprotein N, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene ORF65, which codes for a small capsomere-interacting protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
| GO:0019012: virion |
ECO:0007184: |
C |
Figure 1, lane 4 shows that gene K8.1, which codes for an envelope protein, is present in Kaposi’s Sarcoma-associated Herpesvirus virion |
complete | ||||
Notes
See also
References
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