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PMID:15611275

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Citation

Bora, PS, Sohn, JH, Cruz, JM, Jha, P, Nishihori, H, Wang, Y, Kaliappan, S, Kaplan, HJ and Bora, NS (2005) Role of complement and complement membrane attack complex in laser-induced choroidal neovascularization. J. Immunol. 174:491-7

Abstract

Choroidal neovascularization (CNV), or choroidal angiogenesis, is the hallmark of age-related macular degeneration and a leading cause of visual loss after age 55. The pathogenesis of new choroidal vessel formation is poorly understood. Although inflammation has been implicated in the development of CNV, the role of complement in CNV has not been explored experimentally. A reliable way to produce CNV in animals is to rupture Bruch's membrane with laser photocoagulation. A murine model of laser-induced CNV in C57BL/6 mice revealed the deposition of C3 and membrane attack complex (MAC) in the neovascular complex. CNV was inhibited by complement depletion using cobra venom factor and did not develop in C3(-/-) mice. Anti-murine C6 Abs in C57BL/6 mice inhibited MAC formation and also resulted in the inhibition of CNV. Vascular endothelial growth factor, TGF-beta2, and beta-fibroblast growth factor were elevated in C57BL/6 mice after laser-induced CNV; complement depletion resulted in a marked reduction in the level of these angiogenic factors. Thus, activation of complement, specifically the formation of MAC, is essential for the development of laser- induced choroidal angiogenesis in mice. It is possible that a similar mechanism may be involved in the pathophysiology of other angiogenesis essential diseases.

Links

PubMed

Keywords

Animals; Choroid/blood supply; Choroidal Neovascularization/etiology; Choroidal Neovascularization/metabolism; Choroidal Neovascularization/physiopathology; Complement Activation/physiology; Complement C3/metabolism; Complement Membrane Attack Complex/metabolism; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Fibroblast Growth Factors/metabolism; Immunohistochemistry; Lasers/adverse effects; Male; Mice; Microscopy, Confocal; Reverse Transcriptase Polymerase Chain Reaction; Transforming Growth Factor beta/metabolism; Transforming Growth Factor beta2; Vascular Endothelial Growth Factor A/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CO3

involved_in

GO:0001970: positive regulation of activation of membrane attack complex

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CO3

involved_in

GO:0045766: positive regulation of angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q91X70

involved_in

GO:0001970: positive regulation of activation of membrane attack complex

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q91X70

involved_in

GO:0045917: positive regulation of complement activation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q91X70

involved_in

GO:0045766: positive regulation of angiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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