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PMID:15607976

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Citation

Motamedi, MR, Verdel, A, Colmenares, SU, Gerber, SA, Gygi, SP and Moazed, D (2004) Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs. Cell 119:789-802

Abstract

RNAi-mediated heterochromatin assembly in fission yeast requires the RNA-induced transcriptional silencing (RITS) complex and a putative RNA-directed RNA polymerase (Rdp1). Here we show that Rdp1 is associated with two conserved proteins, Hrr1, an RNA helicase, and Cid12, a member of the polyA polymerase family, in a complex that has RNA-directed RNA polymerase activity (RDRC, RNA-directed RNA polymerase complex). RDRC physically interacts with RITS in a manner that requires the Dicer ribonuclease (Dcr1) and the Clr4 histone methyltransferase. Moreover, both complexes are localized to the nucleus and associate with noncoding centromeric RNAs in a Dcr1-dependent manner. In cells lacking Rdp1, Hrr1, or Cid12, RITS complexes are devoid of siRNAs and fail to localize to centromeric DNA repeats to initiate heterochromatin assembly. These findings reveal a physical and functional link between Rdp1 and RITS and suggest that noncoding RNAs provide a platform for siRNA-dependent localization of RNAi complexes to specific chromosome regions.

Links

PubMed Online version:10.1016/j.cell.2004.11.034

Keywords

Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Nucleus/genetics; Cell Nucleus/metabolism; Centromere/genetics; Centromere/metabolism; Heterochromatin/genetics; Heterochromatin/metabolism; Methyltransferases/genetics; Methyltransferases/metabolism; Protein Binding; RNA Helicases/genetics; RNA Helicases/metabolism; RNA, Fungal/genetics; RNA, Fungal/metabolism; RNA, Untranslated/genetics; RNA, Untranslated/metabolism; RNA-Induced Silencing Complex/genetics; RNA-Induced Silencing Complex/metabolism; Ribonuclease III/genetics; Ribonuclease III/metabolism; Schizosaccharomyces/genetics; Schizosaccharomyces/metabolism; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:RDP1

is_active_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:RDP1

involved_in

GO:0031048: heterochromatin assembly by small RNA

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:RDP1

enables

GO:0003968: RNA-directed 5'-3' RNA polymerase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:RDP1

part_of

GO:0031380: nuclear RNA-directed RNA polymerase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:HRR1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPCC736.11

F

Seeded From UniProt

complete

SCHPO:HRR1

is_active_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:HRR1

involved_in

GO:0031508: pericentric heterochromatin assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:HRR1

involved_in

GO:0031048: heterochromatin assembly by small RNA

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:HRR1

part_of

GO:0031379: RNA-directed RNA polymerase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:HRR1

part_of

GO:0031380: nuclear RNA-directed RNA polymerase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CID12

involved_in

GO:0031508: pericentric heterochromatin assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:CID12

is_active_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CID12

involved_in

GO:0031048: heterochromatin assembly by small RNA

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:CID12

part_of

GO:0031379: RNA-directed RNA polymerase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CID12

part_of

GO:0031380: nuclear RNA-directed RNA polymerase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:AGO1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPCC1739.03

F

Seeded From UniProt

complete


See also

References

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