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PMID:15596717

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Citation

Reinke, CA, Kozik, P and Glick, BS (2004) Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance. Proc. Natl. Acad. Sci. U.S.A. 101:18018-23

Abstract

According to the cisternal maturation hypothesis, endoplasmic reticulum (ER)-derived membranes nucleate new Golgi cisternae. The yeast Saccharomyces cerevisiae offers a unique opportunity to test this idea because small buds contain both ER and Golgi structures early in the cell cycle. We previously predicted that mutants defective in ER inheritance also would show defects in Golgi inheritance. Surprisingly, studies of S. cerevisiae have not revealed the expected link between ER and Golgi inheritance. Here, we revisit this issue by generating mutant strains in which many of the small buds are devoid of detectable ER. These strains also show defects in the inheritance of both early and late Golgi cisternae. Strikingly, virtually all of the buds that lack ER also lack early Golgi cisternae. Our results fit with the idea that membranes exported from the ER coalesce with vesicles derived from existing Golgi compartments to generate new Golgi cisternae. This basic mechanism of Golgi inheritance may be conserved from yeast to vertebrate cells.

Links

PubMed PMC539800 Online version:10.1073/pnas.0408256102

Keywords

Alleles; Bacterial Proteins/metabolism; Carrier Proteins/metabolism; Cell Cycle; Codon; Endoplasmic Reticulum/metabolism; Fungal Proteins/physiology; Golgi Apparatus/metabolism; Golgi Apparatus/physiology; Green Fluorescent Proteins/metabolism; Luminescent Proteins/metabolism; Microscopy, Fluorescence; Mutation; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/metabolism; Time Factors; Vesicular Transport Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SEC8

involved_in

GO:0048313: Golgi inheritance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SEC8

involved_in

GO:0048309: endoplasmic reticulum inheritance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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