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PMID:15583030

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Citation

Wiederkehr, A, De Craene, JO, Ferro-Novick, S and Novick, P (2004) Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J. Cell Biol. 167:875-87

Abstract

The exocyst is an octameric protein complex required to tether secretory vesicles to exocytic sites and to retain ER tubules at the apical tip of budded cells. Unlike the other five exocyst genes, SEC3, SEC5, and EXO70 are not essential for growth or secretion when either the upstream activator rab, Sec4p, or the downstream SNARE-binding component, Sec1p, are overproduced. Analysis of the suppressed sec3Delta, sec5Delta, and exo70Delta strains demonstrates that the corresponding proteins confer differential effects on vesicle targeting and ER inheritance. Sec3p and Sec5p are more critical than Exo70p for ER inheritance. Although nonessential under these conditions, Sec3p, Sec5p, and Exo70p are still important for tethering, as in their absence the exocyst is only partially assembled. Sec1p overproduction results in increased SNARE complex levels, indicating a role in assembly or stabilization of SNARE complexes. Furthermore, a fraction of Sec1p can be coprecipitated with the exoycst. Our results suggest that Sec1p couples exocyst-mediated vesicle tethering with SNARE-mediated docking and fusion.

Links

PubMed PMC2172455 Online version:10.1083/jcb.200408001

Keywords

Cell Division/physiology; Endoplasmic Reticulum/genetics; Endoplasmic Reticulum/metabolism; Exocytosis/physiology; Macromolecular Substances/metabolism; Membrane Fusion/physiology; Munc18 Proteins; Mutation/genetics; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Protein Transport/physiology; SNARE Proteins; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Secretory Vesicles/metabolism; Vesicular Transport Proteins/genetics; Vesicular Transport Proteins/metabolism; Vesicular Transport Proteins/physiology; rab GTP-Binding Proteins/genetics; rab GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:EXO70

involved_in

GO:0001927: exocyst assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:EXO70

involved_in

GO:0006887: exocytosis

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001889
SGD:S000002571

P

Seeded From UniProt

complete

YEAST:SEC3

involved_in

GO:0001927: exocyst assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SEC3

involved_in

GO:0048309: endoplasmic reticulum inheritance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SEC3

involved_in

GO:0048309: endoplasmic reticulum inheritance

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001889
SGD:S000002571

P

Seeded From UniProt

complete

YEAST:SEC5

involved_in

GO:0001927: exocyst assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SEC5

involved_in

GO:0006887: exocytosis

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001889
SGD:S000002571

P

Seeded From UniProt

complete

YEAST:SEC5

involved_in

GO:0048309: endoplasmic reticulum inheritance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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