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PMID:15516325

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Citation

Xu, WP, Shiba, H, Mizuno, N, Uchida, Y, Mouri, Y, Kawaguchi, H and Kurihara, H (2004) Effect of bone morphogenetic proteins-4, -5 and -6 on DNA synthesis and expression of bone-related proteins in cultured human periodontal ligament cells. Cell Biol. Int. 28:675-82

Abstract

Bone morphogenetic proteins (BMPs) have multiple functions in the development and growth of skeletal and extraskeletal tissues. Therefore, BMPs may regulate the regeneration of periodontal tissue. To investigate this issue, we examined the effects of BMP-4, -5 and -6 on DNA synthesis and the expression of bone-related proteins in cultures of human periodontal ligament (HPL) cells. The expression of bone-related proteins was determined by Real-time polymerase chain reaction and enzyme linked immunosorbent assay in cultures of HPL cells. DNA synthesis was estimated by measuring bromoderoxyuridine incorporation. It was found that BMP-4, -5 and -6 enhanced DNA synthesis dose-dependently. BMP-4 and -5 increased the levels of osteopontin, BMP-2, alkaline phosphatase and core binding factor alpha 1 mRNAs. BMP-6 stimulated the expression of osteopontin, BMP-2, ALPase and osteoprotegerin. These findings show that BMP-4, -5 and -6 have different actions on the expression of bone-related proteins and may play a role in the regeneration of periodontal tissue by promoting cell proliferation and protein expression.

Links

PubMed Online version:10.1016/j.cellbi.2004.06.004

Keywords

Bone Morphogenetic Protein 4; Bone Morphogenetic Protein 5; Bone Morphogenetic Protein 6; Bone Morphogenetic Proteins/physiology; Bromodeoxyuridine/metabolism; Cells, Cultured; DNA Replication/physiology; Gene Expression Regulation/physiology; Humans; Periodontal Ligament/cytology; Periodontal Ligament/enzymology; Periodontal Ligament/metabolism; Periodontal Ligament/physiology; RNA, Messenger/biosynthesis; Recombinant Proteins/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TR11B

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:OSTP

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:BMP4

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:P10451)|has_input:(UniProtKB:P05186)|has_input:(UniProtKB:Q13950)|has_input:(UniProtKB:P12643)

Seeded From UniProt

complete

HUMAN:BMP5

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:Q13950)|has_input:(UniProtKB:P10451)|has_input:(UniProtKB:P12643)

Seeded From UniProt

complete

HUMAN:BMP6

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP6

involved_in

GO:0050714: positive regulation of protein secretion

ECO:0000303: author statement without traceable support used in manual assertion

P

  • has_input:(UniProtKB:O00300)|has_input:(UniProtKB:P10451)

Seeded From UniProt

complete

HUMAN:BMP6

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000303: author statement without traceable support used in manual assertion

P

  • has_input:(UniProtKB:P10451)|has_input:(UniProtKB:P12643)|has_input:(UniProtKB:P05186)|has_input:(UniProtKB:O00300)

Seeded From UniProt

complete

HUMAN:BMP5

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

has_regulation_target:(UniProtKB:Q13950)|has_regulation_target:(UniProtKB:P10451)|has_regulation_target:(UniProtKB:P12643)

Seeded From UniProt

complete

HUMAN:BMP5

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP4

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP4

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

has_regulation_target:(UniProtKB:P10451)|has_regulation_target:(UniProtKB:P05186)|has_regulation_target:(UniProtKB:Q13950)|has_regulation_target:(UniProtKB:P12643)

Seeded From UniProt

complete


See also

References

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