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PMID:15509804

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Citation

Pic-Taylor, A, Darieva, Z, Morgan, BA and Sharrocks, AD (2004) Regulation of cell cycle-specific gene expression through cyclin-dependent kinase-mediated phosphorylation of the forkhead transcription factor Fkh2p. Mol. Cell. Biol. 24:10036-46

Abstract

The forkhead transcription factor Fkh2p acts in a DNA-bound complex with Mcm1p and the coactivator Ndd1p to regulate cell cycle-dependent expression of the CLB2 gene cluster in Saccharomyces cerevisiae. Here, we demonstrate that Fkh2p is a target of cyclin-dependent protein kinases and that phosphorylation of Fkh2p promotes interactions between Fkh2p and the coactivator Ndd1p. These phosphorylation-dependent changes in the Fkh2p-Ndd1p complex play an important role in the cell cycle-regulated expression of the CLB2 cluster. Our data therefore identify an important regulatory target for cyclin-dependent kinases in the cell cycle and further our molecular understanding of the key cell cycle regulatory transcription factor Fkh2p.

Links

PubMed PMC525469 Online version:10.1128/MCB.24.22.10036-10046.2004

Keywords

Binding Sites; CDC28 Protein Kinase, S cerevisiae/genetics; CDC28 Protein Kinase, S cerevisiae/metabolism; Cell Cycle; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cyclin B/genetics; Cyclin B/metabolism; Cyclin-Dependent Kinases/genetics; Cyclin-Dependent Kinases/metabolism; Forkhead Transcription Factors; Gene Expression Regulation, Fungal; Genes, Fungal; Macromolecular Substances; Models, Biological; Multigene Family; Mutagenesis; Phosphorylation; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:FKH2

enables

GO:0003712: transcription coregulator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:FKH2

enables

GO:0003712: transcription coregulator activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:FKH2

enables

GO:0001085: RNA polymerase II transcription factor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:FKH2

enables

GO:0001085: RNA polymerase II transcription factor binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:FKH2

enables

GO:0001085: RNA polymerase II transcription factor binding

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001393

F

Seeded From UniProt

complete

YEAST:FKH2

involved_in

GO:0090282: positive regulation of transcription involved in G2/M transition of mitotic cell cycle

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001393

P

Seeded From UniProt

complete


See also

References

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