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PMID:15498891

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Citation

Lee, HY, Zhao, S, Fields, PA and Sherwood, OD (2005) The extent to which relaxin promotes proliferation and inhibits apoptosis of cervical epithelial and stromal cells is greatest during late pregnancy in rats. Endocrinology 146:511-8

Abstract

Relaxin promotes marked growth of the cervix during the second half of rat pregnancy, and this growth is accompanied by an increase in both epithelial and stromal cells. The objective of this study was to test the hypothesis that the extent to which relaxin promotes proliferation and inhibits apoptosis of cervical cells is greatest during late pregnancy in rats. The influence of neutralization of circulating relaxin by iv injection of 5 mg monoclonal antibody against rat relaxin (MCA1) was examined at 3-d intervals throughout the second half of pregnancy. Controls were injected with either 5 mg monoclonal antibody against fluorescein or 0.5 ml PBS vehicle. To evaluate cell proliferation, 5'-bromo-2-deoxyuridine was injected sc 8 h before cervixes were collected. Terminal deoxynucleotidyl transferase-mediated deoxyuridine 5'-triphosphate nick end-labeling and electron microscopy were used to detect apoptotic cells. Neutralization of relaxin with MCA1 decreased the rate of proliferation and increased the rate of apoptosis of cervical cells by d 13. However, the extent to which relaxin influenced these processes was greatest and dramatic by late pregnancy. In MCA1-treated rats on d 22 of pregnancy, the rates of proliferation of both epithelial and stromal cells were less than 20% those in controls, and the rates of apoptosis in epithelial cells and stromal cells were more than 10- and 3-fold, respectively, greater than those in controls. In conclusion, this study provides evidence that the extent to which relaxin promotes proliferation and inhibits apoptosis of cervical epithelial and stromal cells is greatest during late pregnancy.

Links

PubMed Online version:10.1210/en.2004-0796

Keywords

Animals; Apoptosis/physiology; Cell Proliferation; Cervix Uteri/cytology; Cervix Uteri/physiology; Cervix Uteri/ultrastructure; Epithelial Cells/physiology; Epithelial Cells/ultrastructure; Female; Gestational Age; Microscopy, Electron; Pregnancy; Pregnancy, Animal/physiology; Rats; Rats, Sprague-Dawley; Relaxin/physiology; Stromal Cells/cytology; Stromal Cells/physiology; Stromal Cells/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:REL1

involved_in

GO:0042127: regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:REL1

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:REL1

GO:0042127 : regulation of cell proliferation

ECO:0000315:

P

In figure 4, the authors demonstrate that the neutralization of endogenous relaxin during late pregnancy has a negative effect on cervical epithelial and stromal cell proliferation.

complete
CACAO 2042

RAT:REL1

GO:0043066: negative regulation of apoptotic process

ECO:0000315:

P

In figure 6, the authors demonstrate that the neutralization of endogenous relaxin during late pregnancy increases apoptosis in cervical epithelial and stromal tissue.

complete
CACAO 2046


See also

References

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