GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:15489339
| Citation |
Rual, JF, Ceron, J, Koreth, J, Hao, T, Nicot, AS, Hirozane-Kishikawa, T, Vandenhaute, J, Orkin, SH, Hill, DE, van den Heuvel, S and Vidal, M (2004) Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res. 14:2162-8 |
|---|---|
| Abstract |
The recently completed Caenorhabditis elegans genome sequence allows application of high-throughput (HT) approaches for phenotypic analyses using RNA interference (RNAi). As large phenotypic data sets become available, "phenoclustering" strategies can be used to begin understanding the complex molecular networks involved in development and other biological processes. The current HT-RNAi resources represent a great asset for phenotypic profiling but are limited by lack of flexibility. For instance, existing resources do not take advantage of the latest improvements in RNAi technology, such as inducible hairpin RNAi. Here we show that a C. elegans ORFeome resource, generated with the Gateway cloning system, can be used as a starting point to generate alternative HT-RNAi resources with enhanced flexibility. The versatility inherent to the Gateway system suggests that additional HT-RNAi libraries can now be readily generated to perform gene knockdowns under various conditions, increasing the possibilities for phenome mapping in C. elegans. |
| Links |
PubMed PMC528933 Online version:10.1101/gr.2505604 |
| Keywords |
Animals; Caenorhabditis elegans/genetics; Gene Expression Regulation/genetics; Gene Library; Genome; Genotype; Open Reading Frames/physiology; Phenotype; RNA Interference; RNA, Helminth/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
See also
References
See Help:References for how to manage references in GONUTS.
