GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15472079

From GONUTS
Jump to: navigation, search
Citation

Oh, S, Zhang, H, Ludwig, P and van Nocker, S (2004) A mechanism related to the yeast transcriptional regulator Paf1c is required for expression of the Arabidopsis FLC/MAF MADS box gene family. Plant Cell 16:2940-53

Abstract

The Arabidopsis thaliana VERNALIZATION INDEPENDENCE (VIP) gene class has multiple functions in development, including repression of flowering through activation of the MADSbox gene FLC. Epigenetic silencing of FLC plays a substantial role in the promotion of flowering through cold (vernalization). To better understand how VIP genes influence development, we undertook a genetic and molecular study of the previously uncharacterized VIP5 and VIP6 genes. We found that loss of function of these genes also resulted in downregulation of other members of the FLC/MAF gene family, including the photoperiodic pathway regulator MAF1/FLM. We cloned VIP5 and VIP6 through mapping and transcriptional profiling. Both proteins are closely related to distinct components of budding yeast Paf1C, a transcription factor that assists in establishment and maintenance of transcription-promotive chromatin modifications such as ubiquitination of H2B by Bre1/Rad6 and methylation of histone H3 lysine-4 by the trithorax-related histone methylase Set1. Genetic analysis and coimmunoprecipitation experiments suggest that VIP5 and VIP6 function in the same mechanism as the previously described VIP3 and VIP4. Our findings suggest that an evolutionarily conserved transcriptional mechanism plays an essential role in the maintenance of gene expression in higher eukaryotes and has a central function in flowering.

Links

PubMed PMC527190 Online version:10.1105/tpc.104.026062

Keywords

Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/isolation & purification; Arabidopsis Proteins/metabolism; Down-Regulation/genetics; Flowers/genetics; Flowers/metabolism; Gene Expression Regulation, Plant/genetics; Gene Silencing/physiology; Genes, Regulator/genetics; Histones/metabolism; MADS Domain Proteins/genetics; MADS Domain Proteins/metabolism; Matrix Attachment Region Binding Proteins/genetics; Matrix Attachment Region Binding Proteins/metabolism; Methylation; Nuclear Proteins/genetics; Plant Proteins/genetics; Plant Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Transcription Factors/genetics; Transcription Factors/isolation & purification; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:VIP6

acts_upstream_of_or_within

GO:0009910: negative regulation of flower development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:VIP6

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:VIP5

acts_upstream_of_or_within

GO:0009910: negative regulation of flower development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:VIP5

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.