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PMID:15458422

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Citation

Narumi, I, Satoh, K, Cui, S, Funayama, T, Kitayama, S and Watanabe, H (2004) PprA: a novel protein from Deinococcus radiodurans that stimulates DNA ligation. Mol. Microbiol. 54:278-85

Abstract

The extraordinary radiation resistance of Deinococcus radiodurans results from the efficient capacity of the bacterium to repair DNA double-strand breaks. By analysing the DNA damage repair-deficient mutant, KH311, a unique radiation-inducible gene (designated pprA) responsible for loss of radiation resistance was identified. Investigations in vitro showed that the gene product of pprA (PprA) preferentially bound to double-stranded DNA carrying strand breaks, inhibited Escherichia coli exonuclease III activity, and stimulated the DNA end-joining reaction catalysed by ATP-dependent and NAD-dependent DNA ligases. These results suggest that D. radiodurans has a radiation-induced non-homologous end-joining repair mechanism in which PprA plays a critical role.

Links

PubMed Online version:10.1111/j.1365-2958.2004.04272.x

Keywords

Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA/genetics; DNA/metabolism; DNA Damage; DNA Ligases/genetics; DNA Ligases/metabolism; DNA Repair; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Deinococcus/genetics; Deinococcus/metabolism; Deinococcus/radiation effects; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mutation; Rec A Recombinases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DEIRA:PPRA

involved_in

GO:0051106: positive regulation of DNA ligation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DEIRA:PPRA

involved_in

GO:0006281: DNA repair

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DEIRA:PPRA

NOT|enables

GO:0003697: single-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DEIRA:PPRA

enables

GO:0003690: double-stranded DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DEIRA:PPRA

enables

GO:0003684: damaged DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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