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PMID:1538784

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Citation

Hagan, I and Yanagida, M (1992) Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast. Nature 356:74-6

Abstract

Several mitotic and meiotic gene products are related to the microtubule motor kinesin, providing insight into the molecular basis of the complex motile events responsible for spindle formation and function. Of these genes, three have been shown to affect spindle structure when mutated. The most severe phenotype is seen in Aspergillus nidulans bimC and Schizosaccharomyces pombe cut7 mutants. In both fungi the intranuclear spindle is bipolar, with microtubules that emanate from spindle pole bodies at either pole, interdigitating in a central overlap zone. In bimC and cut7 mutants, microtubule interdigitation does not appear to take place, instead two unconnected half spindles form and chromosome separation fails. Here we report that cut7 protein concentrates on or near the spindle pole bodies throughout mitotic and meiotic nuclear division and associates with mitotic spindle microtubules in a stage-specific manner, associating with the mid-anaphase B midzone. In cut7ts mutants, spindle pole bodies stain but mitotic microtubules do not.

Links

PubMed Online version:10.1038/356074a0

Keywords

Anaphase/genetics; Aspergillus nidulans/metabolism; Fluorescent Antibody Technique; Fungal Proteins/metabolism; Kinesin/metabolism; Meiosis/genetics; Microtubules/metabolism; Mitosis/genetics; Mutation/genetics; Nuclear Proteins/metabolism; Schizosaccharomyces/metabolism; Spindle Apparatus/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:CUT7

involved_in

GO:0000073: initial mitotic spindle pole body separation

ECO:0000269: experimental evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:CUT7

is_active_in

GO:0090619: meiotic spindle pole

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CUT7

is_active_in

GO:0044732: mitotic spindle pole body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CUT7

is_active_in

GO:1990023: mitotic spindle midzone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CUT7

is_active_in

GO:0072687: meiotic spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CUT7

part_of

GO:0044732: mitotic spindle pole body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CUT7

part_of

GO:0072687: meiotic spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CUT7

part_of

GO:1990023: mitotic spindle midzone

ECO:0000314: direct assay evidence used in manual assertion

C

exists_during:(GO:0000092)

Seeded From UniProt

complete

Notes

See also

References

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