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PMID:15383546
Citation |
Wenstrup, RJ, Florer, JB, Brunskill, EW, Bell, SM, Chervoneva, I and Birk, DE (2004) Type V collagen controls the initiation of collagen fibril assembly. J. Biol. Chem. 279:53331-7 |
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Abstract |
Vertebrate collagen fibrils are heterotypically composed of a quantitatively major and minor fibril collagen. In non-cartilaginous tissues, type I collagen accounts for the majority of the collagen mass, and collagen type V, the functions of which are poorly understood, is a minor component. Type V collagen has been implicated in the regulation of fibril diameter, and we reported recently preliminary evidence that type V collagen is required for collagen fibril nucleation (Wenstrup, R. J., Florer, J. B., Cole, W. G., Willing, M. C., and Birk, D. E. (2004) J. Cell. Biochem. 92, 113-124). The purpose of this study was to define the roles of type V collagen in the regulation of collagen fibrillogenesis and matrix assembly. Mouse embryos completely deficient in pro-alpha1(V) chains were created by homologous recombination. The col5a1-/- animals die in early embryogenesis, at approximately embryonic day 10. The type V collagen-deficient mice demonstrate a virtual lack of collagen fibril formation. In contrast, the col5a1+/- animals are viable. The reduced type V collagen content is associated with a 50% reduction in fibril number and dermal collagen content. In addition, relatively normal, cylindrical fibrils are assembled with a second population of large, structurally abnormal collagen fibrils. The structural properties of the abnormal matrix are decreased relative to the wild type control animals. These data indicate a central role for the evolutionary, ancient type V collagen in the regulation of fibrillogenesis. The complete dependence of fibril formation on type V collagen is indicative of the critical role of the latter in early fibril initiation. In addition, this fibril collagen is important in the determination of fibril structure and matrix organization. |
Links |
PubMed Online version:10.1074/jbc.M409622200 |
Keywords |
Animals; Blotting, Western; Cell Culture Techniques; Collagen/chemistry; Collagen/metabolism; Collagen Type V/chemistry; Collagen Type V/physiology; DNA/metabolism; Electrophoresis, Polyacrylamide Gel; Embryo, Mammalian/metabolism; Exons; Extracellular Matrix/metabolism; Genetic Vectors; Genotype; In Situ Hybridization; Mice; Microscopy, Electron; Microscopy, Electron, Transmission; Models, Biological; Models, Genetic; Skin/metabolism; Time Factors |
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