GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15358627

From GONUTS
Jump to: navigation, search
Citation

Li, Y, McClintick, J, Zhong, L, Edenberg, HJ, Yoder, MC and Chan, RJ (2005) Murine embryonic stem cell differentiation is promoted by SOCS-3 and inhibited by the zinc finger transcription factor Klf4. Blood 105:635-7

Abstract

Embryonic stem (ES) cells homozygous for a Shp-2 mutation (Shp-2(Delta46-110)) demonstrate leukemia inhibitory factor (LIF) hypersensitivity and increased LIF-stimulated phosphorylation of signal transducer and activator of transcription (STAT3). We hypothesized that LIF-responsive genes in Shp-2(Delta46-110) cells would represent potential candidates for molecules vital for ES cell self-renewal. Using microarray analysis, we detected 41 genes whose expression was modified by LIF in Shp-2(Delta46-110) ES cells. Induction of 2 significantly up-regulated genes, suppressor of cytokine signaling-3 (SOCS-3) and Kruppel-like factor 4 (Klf4), was verified using Northern blotting. ES cells overexpressing SOCS-3 had an increased capacity to differentiate to hematopoietic progenitors, rather than to self-renew. In contrast, ES cells overexpressing Klf4 had a greater capacity to self-renew based on secondary embryoid body (EB) formation. Klf4-transduced d6 EBs expressed higher levels of Oct-4, consistent with the notion that Klf4 promotes ES cell self-renewal. These findings verify the negative role of SOCS-3 on LIF signaling and provide a novel role for Klf4 in ES cell function.

Links

PubMed Online version:10.1182/blood-2004-07-2681

Keywords

Animals; Cell Differentiation/physiology; DNA-Binding Proteins/genetics; Gene Expression Regulation, Developmental; Kruppel-Like Transcription Factors; Muridae; Oligonucleotide Array Sequence Analysis; Repressor Proteins/genetics; Signal Transduction/physiology; Stem Cells/cytology; Stem Cells/physiology; Suppressor of Cytokine Signaling Proteins; Transcription Factors/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SOCS3

involved_in

GO:0045597: positive regulation of cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOCS3

involved_in

GO:0030154: cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SOCS3

involved_in

GO:0045597: positive regulation of cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:96787

P

Seeded From UniProt

complete

MOUSE:SOCS3

involved_in

GO:0009968: negative regulation of signal transduction

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:96787

P

Seeded From UniProt

complete

MOUSE:SOCS3

involved_in

GO:0030154: cell differentiation

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:96787

P

Seeded From UniProt

complete

MOUSE:SOCS3

involved_in

GO:0007165: signal transduction

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:96787

P

Seeded From UniProt

complete

MOUSE:LIF

involved_in

GO:0035019: somatic stem cell population maintenance

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:LIF

involved_in

GO:0035019: somatic stem cell population maintenance

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1201791

P

Seeded From UniProt

complete

MOUSE:LIF

involved_in

GO:0035019: somatic stem cell population maintenance

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1342287

P

Seeded From UniProt

complete

MOUSE:KLF4

involved_in

GO:0035019: somatic stem cell population maintenance

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:KLF4

acts_upstream_of_or_within

GO:0019827: stem cell population maintenance

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.