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PMID:15304217

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Citation

Walton, TA and Sousa, MC (2004) Crystal structure of Skp, a prefoldin-like chaperone that protects soluble and membrane proteins from aggregation. Mol. Cell 15:367-74

Abstract

The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. Here we report the crystal structure of Skp from E. coli. The structure of the Skp trimer resembles a jellyfish with alpha-helical tentacles protruding from a beta barrel body defining a central cavity. The architecture of Skp is unexpectedly similar to that of Prefoldin/GimC, a cytosolic chaperone present in eukaria and archea, that binds unfolded substrates in its central cavity. The ability of Skp to prevent the aggregation of model substrates in vitro is independent of ATP. Skp can interact directly with membrane lipids and lipopolysaccharide (LPS). These interactions are needed for efficient Skp-assisted folding of membrane proteins. We have identified a putative LPS binding site on the outer surface of Skp and propose a model for unfolded substrate binding.

Links

PubMed Online version:10.1016/j.molcel.2004.07.023

Keywords

Adenosine Triphosphate/metabolism; Amino Acid Sequence; Bacterial Outer Membrane Proteins/metabolism; Binding Sites; Crystallography, X-Ray; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; Escherichia coli/chemistry; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Lipopolysaccharides/metabolism; Molecular Chaperones/chemistry; Molecular Chaperones/metabolism; Molecular Sequence Data; Protein Folding; Protein Structure, Tertiary; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:SKP

acts_upstream_of_or_within

GO:0050821: protein stabilization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SKP

enables

GO:0001530: lipopolysaccharide binding

ECO:0000245: automatically integrated combinatorial evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:SKP

involved_in

GO:0050821: protein stabilization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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