GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15269104

From GONUTS
Jump to: navigation, search
Citation

Johnson, AL, Bridgham, JT and Woods, DC (2004) Cellular mechanisms and modulation of activin A- and transforming growth factor beta-mediated differentiation in cultured hen granulosa cells. Biol. Reprod. 71:1844-51

Abstract

Undifferentiated granulosa cells from prehierarchal (6- to 8-mm-diameter) hen follicles express very low to undetectable levels of LH receptor (LH-R) mRNA, P450 cholesterol side chain cleavage (P450scc) enzyme activity, and steroidogenic acute regulatory (StAR) protein, and produce negligible progesterone, in vitro, following an acute (3-h) challenge with either FSH or LH. It has previously been established that culturing such cells with FSH for 18-20 h induces LH-R, P450scc, and StAR expression, which enables the initiation of progesterone production. The present studies were conducted to characterize the ability of activin and transforming growth factor (TGF) beta, both alone and in combination with FSH, to promote hen granulosa cell differentiation, in vitro. A 20-h culture of prehierarchal follicle granulosa cells with activin A or transforming growth factor beta (TGFbeta)1 increased LH-R mRNA levels compared with control cultured cells. Activin A and TGFbeta1 also promoted FSH-receptor (FSH-R) mRNA expression when combined with FSH treatment. Neither activin A nor TGFbeta1 alone stimulated progesterone production after 20 h culture. However, preculture with either factor for 20 h (to induce gonadotropin receptor mRNA expression) followed by a 3-h challenge with FSH or LH potentiated StAR expression and progesterone production compared with cells challenged with gonadotropin in the absence of activin A or TGFbeta1 preculture. Significantly, activation of the mitogen-activated protein (MAP) kinase pathway with transforming growth factor alpha (TGFalpha) (monitored by Erk phosphorylation) blocked TGFbeta1-induced LH-R expression, and this effect was associated with the inhibition of Smad2 phosphorylation. We conclude that a primary differentiation-inducing action of activin A and TGFbeta1 on hen granulosa cells from prehierarchal follicles is directed toward LH-R expression. Enhanced LH-R levels subsequently sensitize granulosa cells to LH, which in turn promotes StAR plus P450scc expression and subsequently an increase in P4 production. Significantly, the finding that TGFbeta signaling is negatively regulated by MAP kinase signaling is proposed to represent a mechanism that prevents premature differentiation of granulosa cells.

Links

PubMed Online version:10.1095/biolreprod.104.032573

Keywords

Activins/pharmacology; Activins/physiology; Animals; Cell Differentiation/drug effects; Cell Differentiation/physiology; Cells, Cultured; Chickens; Drug Combinations; Female; Follicle Stimulating Hormone/pharmacology; Granulosa Cells/cytology; Humans; Inhibin-beta Subunits/pharmacology; Inhibin-beta Subunits/physiology; Mitogen-Activated Protein Kinases/metabolism; Recombinant Proteins/pharmacology; Signal Transduction/physiology; Transforming Growth Factor beta/pharmacology; Transforming Growth Factor beta/physiology; Transforming Growth Factor beta1

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:FSHR

involved_in

GO:0033686: positive regulation of luteinizing hormone secretion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:TGFB1

involved_in

GO:0033686: positive regulation of luteinizing hormone secretion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:TGFB1

involved_in

GO:0042327: positive regulation of phosphorylation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:TGFB1

involved_in

GO:0042701: progesterone secretion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:TGFB1

involved_in

GO:0046881: positive regulation of follicle-stimulating hormone secretion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:TGFB1

involved_in

GO:0050708: regulation of protein secretion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q6J1M9

involved_in

GO:0010468: regulation of gene expression

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q90ZK6

P

Seeded From UniProt

complete

CHICK:Q6J1M9

involved_in

GO:0042326: negative regulation of phosphorylation

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q90ZK6

P

Seeded From UniProt

complete

CHICK:Q6J1M9

involved_in

GO:0042326: negative regulation of phosphorylation

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P09531

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.