PMID:15263013

Citation	Reyes-Grajeda, JP, Moreno, A and Romero, A (2004) Crystal structure of ovocleidin-17, a major protein of the calcified Gallus gallus eggshell: implications in the calcite mineral growth pattern. J. Biol. Chem. 279:40876-81
Abstract	Ovocleidin-17 (OC17) from Gallus gallus is one of the best candidates to control and regulate the deposition of calcium carbonate in the calcified eggshell layer. Here, the crystal structure of monomeric OC17, determined at a resolution of 1.5 A, was refined to a crystallographic R-factor of 20.1%. This is the first protein directly involved in a non-pathological biomineralization process resolved by x-ray diffraction to date. The protein has a mixed alpha/beta structure containing a single C-type lectin-like domain. However, although OC17 shares the conserved scaffold of the C-type lectins, it does not bind carbohydrates. Nevertheless, in vitro OC17 modifies the crystalline habit of calcium carbonate (CaCO3) and the pattern of crystal growth at intervals of 5-200 microg/ml. Determining the three-dimensional structure of OC17 contributes to a better understanding of the biological behavior of structurally related biomolecules and of the mechanisms involved in eggshell and other mineralization processes.
Links	PubMed Online version:10.1074/jbc.M406033200
Keywords	Amino Acid Sequence; Animals; Binding Sites; Calcium Carbonate/chemistry; Carbohydrates/chemistry; Chickens; Crystallography, X-Ray; Cysteine/chemistry; Egg Proteins/chemistry; Egg Shell/chemistry; Lectins/chemistry; Microscopy, Electron, Scanning; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Static Electricity; X-Ray Diffraction
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CHICK:OC17	located_in	GO:0005615: extracellular space	ECO:0000304: author statement supported by traceable reference used in manual assertion		C		Seeded From UniProt	complete
CHICK:OC17	enables	GO:0043167: ion binding	ECO:0000304: author statement supported by traceable reference used in manual assertion		F		Seeded From UniProt	complete
CHICK:OC17	part_of	GO:0005615: extracellular space	ECO:0000304: author statement supported by traceable reference used in manual assertion		C		Seeded From UniProt	complete
CHICK:OC17	involved_in	GO:0031214: biomineral tissue development	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
CHICK:OC17	NOT|enables	GO:0005509: calcium ion binding	ECO:0000255: match to sequence model evidence used in manual assertion		F		Seeded From UniProt	Missing: with/from

References

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