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PMID:15263013

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Citation

Reyes-Grajeda, JP, Moreno, A and Romero, A (2004) Crystal structure of ovocleidin-17, a major protein of the calcified Gallus gallus eggshell: implications in the calcite mineral growth pattern. J. Biol. Chem. 279:40876-81

Abstract

Ovocleidin-17 (OC17) from Gallus gallus is one of the best candidates to control and regulate the deposition of calcium carbonate in the calcified eggshell layer. Here, the crystal structure of monomeric OC17, determined at a resolution of 1.5 A, was refined to a crystallographic R-factor of 20.1%. This is the first protein directly involved in a non-pathological biomineralization process resolved by x-ray diffraction to date. The protein has a mixed alpha/beta structure containing a single C-type lectin-like domain. However, although OC17 shares the conserved scaffold of the C-type lectins, it does not bind carbohydrates. Nevertheless, in vitro OC17 modifies the crystalline habit of calcium carbonate (CaCO3) and the pattern of crystal growth at intervals of 5-200 microg/ml. Determining the three-dimensional structure of OC17 contributes to a better understanding of the biological behavior of structurally related biomolecules and of the mechanisms involved in eggshell and other mineralization processes.

Links

PubMed Online version:10.1074/jbc.M406033200

Keywords

Amino Acid Sequence; Animals; Binding Sites; Calcium Carbonate/chemistry; Carbohydrates/chemistry; Chickens; Crystallography, X-Ray; Cysteine/chemistry; Egg Proteins/chemistry; Egg Shell/chemistry; Lectins/chemistry; Microscopy, Electron, Scanning; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Static Electricity; X-Ray Diffraction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:OC17

located_in

GO:0005615: extracellular space

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:OC17

enables

GO:0043167: ion binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CHICK:OC17

part_of

GO:0005615: extracellular space

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

CHICK:OC17

involved_in

GO:0031214: biomineral tissue development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:OC17

NOT|enables

GO:0005509: calcium ion binding

ECO:0000255: match to sequence model evidence used in manual assertion

F

Seeded From UniProt

Missing: with/from


See also

References

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