GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15258578

From GONUTS
Jump to: navigation, search
Citation

Jackson, SH, Devadas, S, Kwon, J, Pinto, LA and Williams, MS (2004) T cells express a phagocyte-type NADPH oxidase that is activated after T cell receptor stimulation. Nat. Immunol. 5:818-27

Abstract

T cell receptor (TCR) stimulation induces rapid generation of reactive oxygen species, although the mechanisms for this are unclear. Here we found that T cells expressed a functional phagocyte-type nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. TCR crosslinking induced oxidase activation through the recruitment of preformed Fas ligand and Fas. TCR stimulation induced three separable events generating reactive oxygen species: rapid hydrogen peroxide production independent of Fas or NADPH oxidase; sustained hydrogen peroxide production dependent on both Fas and NADPH oxidase; and delayed superoxide production that was dependent on Fas ligand and Fas yet independent of NADPH oxidase. NADPH oxidase-deficient T cells showed enhanced activation of the kinase Erk and a relative increase in T helper type 1 cytokine secretion. Thus, mature T cells express a phagocyte-type NADPH oxidase that regulates elements of TCR signaling.

Links

PubMed Online version:10.1038/ni1096

Keywords

Animals; Antigens, CD95; Cells, Cultured; Fas Ligand Protein; Humans; Immunoblotting; Lymphocyte Activation/immunology; Membrane Glycoproteins; NADPH Oxidase/metabolism; Reactive Oxygen Species/immunology; Reactive Oxygen Species/metabolism; Receptors, Antigen, T-Cell/immunology; Receptors, Antigen, T-Cell/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction/immunology; T-Lymphocytes/enzymology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NCF1

acts_upstream_of_or_within

GO:0050665: hydrogen peroxide biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1934143

P

  • occurs_in:(CL:0000084)

Seeded From UniProt

complete

MOUSE:CY24B

acts_upstream_of_or_within

GO:0050665: hydrogen peroxide biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857284

P

  • occurs_in:(CL:0000084)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.