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PMID:15256501

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Citation

Amit, I, Yakir, L, Katz, M, Zwang, Y, Marmor, MD, Citri, A, Shtiegman, K, Alroy, I, Tuvia, S, Reiss, Y, Roubini, E, Cohen, M, Wides, R, Bacharach, E, Schubert, U and Yarden, Y (2004) Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding. Genes Dev. 18:1737-52

Abstract

The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

Links

PubMed PMC478194 Online version:10.1101/gad.294904

Keywords

Base Sequence; Binding Sites; Cells, Cultured; Cytoplasm/metabolism; Cytoplasm/physiology; DNA-Binding Proteins/metabolism; Endocytosis/physiology; Endosomal Sorting Complexes Required for Transport; Exocytosis/physiology; Fluorescent Antibody Technique; Gene Library; Gene Products, gag/metabolism; HIV/metabolism; Humans; Molecular Sequence Data; Peptide Fragments; Plasmids/genetics; Protein Transport/physiology; RNA, Small Interfering/genetics; Receptor, Epidermal Growth Factor/metabolism; Sequence Analysis, DNA; Transcription Factors/metabolism; Transfection; Two-Hybrid System Techniques; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/metabolism; Ubiquitins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:TS101

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q6UWE0

F

Seeded From UniProt

complete

MOUSE:TS101

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:TS101

involved_in

GO:0046755: viral budding

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:LRSM1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q61187

F

Seeded From UniProt

complete

HUMAN:LRSM1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:LRSM1

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:LRSM1

enables

GO:0004842: ubiquitin-protein transferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:LRSM1

involved_in

GO:0051865: protein autoubiquitination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:LRSM1

involved_in

GO:0070086: ubiquitin-dependent endocytosis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:LRSM1

involved_in

GO:0046755: viral budding

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:LRSM1

involved_in

GO:0045806: negative regulation of endocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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