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PMID:15247238

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Citation

Kissová, I, Deffieu, M, Manon, S and Camougrand, N (2004) Uth1p is involved in the autophagic degradation of mitochondria. J. Biol. Chem. 279:39068-74

Abstract

The absence of the outer mitochondrial membrane protein Uth1p was found to induce resistance to rapamycin treatment and starvation, two conditions that induce the autophagic process. Biochemical studies showed the onset of a fully active autophagic activity both in wild-type and Deltauth1 strains. On the other hand, the disorganization of the mitochondrial network induced by rapamycin treatment or 15 h of nitrogen starvation was followed in cells expressing mitochondria-targeted green fluorescent protein; a rapid colocalization of green fluorescent protein fluorescence with vacuole-selective FM4-64 labeling was observed in the wild-type but not in the Deltauth1 strain. Degradation of mitochondrial proteins, followed by Western blot analysis, did not occur in mutant strains carrying null mutations of the vacuolar protease Pep4p, the autophagy-specific protein Atg5p, and Uth1p. These data show that, although the autophagic machinery was fully functional in the absence of Uth1p, this protein is involved in the autophagic degradation of mitochondria.

Links

PubMed Online version:10.1074/jbc.M406960200

Keywords

Alkaline Phosphatase/metabolism; Aspartic Acid Endopeptidases/metabolism; Autophagy; Blotting, Western; Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology; Cycloheximide/pharmacology; Green Fluorescent Proteins; Heat-Shock Proteins/metabolism; Heat-Shock Proteins/physiology; Intracellular Membranes/metabolism; Luminescent Proteins/metabolism; Membrane Proteins; Microscopy, Fluorescence; Mitochondria/metabolism; Mitochondrial Proteins; Nitrogen/metabolism; Plasmids/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Sirolimus/pharmacology; Spectrometry, Fluorescence; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:UTH1

involved_in

GO:0000422: autophagy of mitochondrion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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