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PMID:15231775

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Citation

van Ooij, C, Eichenberger, P and Losick, R (2004) Dynamic patterns of subcellular protein localization during spore coat morphogenesis in Bacillus subtilis. J. Bacteriol. 186:4441-8

Abstract

Endospores of Bacillus subtilis are encased in a thick, proteinaceous shell known as the coat, which is composed of a large number of different proteins. Here we report the identification of three previously uncharacterized coat-associated proteins, YabP, YheD, and YutH, and their patterns of subcellular localization during the process of sporulation, obtained by using fusions of the proteins to the green fluorescent protein (GFP). YabP-GFP was found to form both a shell and a ring around the center of the forespore across the short axis of the sporangium. YheD-GFP, in contrast, formed two rings around the forespore that were offset from its midpoint, before it eventually redistributed to form a shell around the developing spore. Finally, YutH-GFP initially localized to a focus at one end of the forespore, which then underwent transformation into a ring that was located adjacent to the forespore. Next, the ring became a cap at the mother cell pole of the forespore that eventually spread around the entire developing spore. Thus, each protein exhibited its own distinct pattern of subcellular localization during the course of coat morphogenesis. We concluded that spore coat assembly is a dynamic process involving diverse patterns of protein assembly and localization.

Links

PubMed PMC438564 Online version:10.1128/JB.186.14.4441-4448.2004

Keywords

Bacillus subtilis/chemistry; Bacillus subtilis/metabolism; Bacillus subtilis/physiology; Bacillus subtilis/ultrastructure; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Compartmentation; Cell Membrane/metabolism; Cytoplasm/metabolism; Genes, Bacterial; Genes, Reporter; Green Fluorescent Proteins; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Microscopy, Fluorescence; Morphogenesis; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Spores, Bacterial/chemistry; Spores, Bacterial/growth & development; Spores, Bacterial/metabolism; Spores, Bacterial/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BACSU:YABP

GO:0042601: endospore-forming forespore

ECO:0000314:

C

Figure 1 A shows location of yabP in the forespore membrane

complete
CACAO 2590

BACSU:YABP

part_of

GO:0042601: endospore-forming forespore

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BACSU:YHED

part_of

GO:0042601: endospore-forming forespore

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BACSU:YHED

GO:0042601: endospore-forming forespore

ECO:0000314:

C

Figure 4 shows location of Yhe-D in forespore membrane

complete
CACAO 2591

BACSU:YUTH

part_of

GO:0042601: endospore-forming forespore

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BACSU:YUTH

GO:0042601: endospore-forming forespore

ECO:0000314:

C

Figure 6. Membrane.

complete
CACAO 2592


See also

References

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