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PMID:15225635

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Citation

Oksvold, MP, Huitfeldt, HS and Langdon, WY (2004) Identification of 14-3-3zeta as an EGF receptor interacting protein. FEBS Lett. 569:207-10

Abstract

The 14-3-3 proteins are known to interact with a number of proteins involved in the regulation of cell signaling. Here, we describe an association of 14-3-3zeta with the epidermal growth factor receptor (EGFR) that is rapidly induced by EGF. The 1028-EGFR truncated mutant which lacks the cytoplasmic tail from amino acids 1029-1186 identified the binding site for 14-3-3 to be between amino acid 1028 and the receptor carboxyl terminus. Mutational deletion of serine residues 1046, 1047, 1057 and 1142 did not inhibit EGF-induced 14-3-3 association with the receptor. Immunofluorescence microscopy indicated an EGF-induced co-localization of EGFR and HA-14-3-3zeta along the plasma membrane. Our finding adds to the growing complexity of EGF receptor signaling and indicates a role for 14-3-3 proteins in EGF receptor signaling or regulation.

Links

PubMed Online version:10.1016/j.febslet.2004.05.068

Keywords

14-3-3 Proteins; 3T3 Cells; Amino Acid Sequence; Animals; Binding Sites; Conserved Sequence; Epidermal Growth Factor/pharmacology; HeLa Cells; Humans; Kinetics; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Phospholipases A/metabolism; Receptor, Epidermal Growth Factor/genetics; Receptor, Epidermal Growth Factor/metabolism; Recombinant Proteins/metabolism; Transfection; Tyrosine 3-Monooxygenase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:EGFR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P63104

F

Seeded From UniProt

complete

HUMAN:1433Z

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P00533

F

Seeded From UniProt

complete

HUMAN:EGFR_original

GO:0005515: protein binding

IPI: Inferred from Physical Interaction: UniProtKB:P63104

F


See also

References

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