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PMID:15193767

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Citation

Wang, X, Li, X, Wang, K, Zhou, H, Xue, B, Li, L and Wang, X (2004) Forskolin cooperating with growth factor on generation of dopaminergic neurons from human fetal mesencephalic neural progenitor cells. Neurosci. Lett. 362:117-21

Abstract

Forskolin was tested for its co-activating ability to enhance the function of fibroblast growth factor (FGF) 8 on dopaminergic (DAergic) differentiation from human fetal mesencephalic neural progenitor cells (NPCs). When NPCs were treated with FGF8 alone, the DAergic phenotype was expressed lightly. The addition of 10 microM forskolin increased the number of DAergic neurons, cooperating with 50 ng/ml FGF8. These cells produced neurotransmitter DA, which was measured by high-performance liquid chromatography. Reverse transcriptase-polymerase chain reaction analysis demonstrated that differentiated cells expressed DAergic development-relative genes tyrosine hydroxylase (TH), nuclear receptor-related factor 1 (Nurr1) and D2 receptor (D2R), indicating that matured DAergic neurons could be obtained under these present conditions. The results suggest that forskolin plus FGF8 may contribute to more efficient production of DAergic neurons from human-derived NPCs for therapy of neurodegenerative diseases.

Links

PubMed Online version:10.1016/j.neulet.2004.03.007

Keywords

Cell Differentiation/drug effects; Cell Differentiation/physiology; Cells, Cultured; Dopamine/biosynthesis; Dopamine/genetics; Dose-Response Relationship, Drug; Fetus; Fibroblast Growth Factor 8; Fibroblast Growth Factors/pharmacology; Forskolin/pharmacology; Humans; Mesencephalon/cytology; Mesencephalon/drug effects; Mesencephalon/metabolism; Neurons/cytology; Neurons/drug effects; Neurons/metabolism; Stem Cells/cytology; Stem Cells/drug effects; Stem Cells/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:FGF8

involved_in

GO:0071542: dopaminergic neuron differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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