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PMID:15190063

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Citation

Alexander, DE, Kaczorowski, DJ, Jackson-Fisher, AJ, Lowery, DM, Zanton, SJ and Pugh, BF (2004) Inhibition of TATA binding protein dimerization by RNA polymerase III transcription initiation factor Brf1. J. Biol. Chem. 279:32401-6

Abstract

The Brf1 subunit of TFIIIB plays an important role in recruiting the TATA-binding protein (TBP) to the up-stream region of genes transcribed by RNA polymerase III. When TBP is not bound to promoters, it sequesters its DNA binding domain through dimerization. Promoter assembly factors therefore might be required to dissociate TBP into productively binding monomers. Here we show that Saccharomyces cerevisiae Brf1 induces TBP dimers to dissociate. The high affinity TBP binding domain of Brf1 is not sufficient to promote TBP dimer dissociation but in addition requires the TFIIB homology domain of Brf1. A model is proposed to explain how two distinct functional domains of Brf1 work in concert to dissociate TBP into monomers.

Links

PubMed Online version:10.1074/jbc.M405782200

Keywords

Alleles; Cross-Linking Reagents/pharmacology; Dimerization; Gene Deletion; Glutathione Transferase/metabolism; Humans; Models, Biological; Mutation; Plasmids/metabolism; Promoter Regions, Genetic; Protein Binding; Protein Structure, Tertiary; RNA Polymerase III/metabolism; Recombinant Proteins/chemistry; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; TATA-Binding Protein Associated Factors; TATA-Box Binding Protein/chemistry; Time Factors; Transcription Factor TFIIIB/chemistry; Transcription Factor TFIIIB/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:TF3B

enables

GO:0017025: TBP-class protein binding

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(SGD:S000000950)

Seeded From UniProt

complete


See also

References

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