GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15155734

From GONUTS
Jump to: navigation, search
Citation

Tikhonova, EB and Zgurskaya, HI (2004) AcrA, AcrB, and TolC of Escherichia coli Form a Stable Intermembrane Multidrug Efflux Complex. J. Biol. Chem. 279:32116-24

Abstract

Many transporters of Gram-negative bacteria involved in the extracellular secretion of proteins and the efflux of toxic molecules operate by forming intermembrane complexes. These complexes are proposed to span both inner and outer membranes and create a bridge across the periplasm. In this study, we analyzed interactions between the inner and outer membrane components of the tri-partite multidrug efflux pump AcrAB-TolC from Escherichia coli. We found that, once assembled, the intermembrane AcrAB-TolC complex is stable during the separation of the inner and outer membranes and subsequent purification. All three components of the complex co-purify when the affinity tag is attached to either of the proteins suggesting bi-partite interactions between AcrA, AcrB, and TolC. We show that antibiotics, the substrates of AcrAB-TolC, stabilize interactions within the complex. However, the formation of the AcrAB-TolC complex does not require an input of energy.

Links

PubMed Online version:10.1074/jbc.M402230200

Keywords

Carrier Proteins/metabolism; Cell Division; Cell Membrane/metabolism; Centrifugation, Density Gradient; Cross-Linking Reagents/pharmacology; Drug Resistance, Multiple; Electrophoresis, Polyacrylamide Gel; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Lipoproteins/metabolism; Membrane Proteins/metabolism; Membrane Transport Proteins; Multidrug Resistance-Associated Proteins; Nuclear Proteins/metabolism; Plasmids/metabolism; Protein Structure, Tertiary; Sucrose/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:TOLC

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P0AE06

F

Seeded From UniProt

complete

ECOLI:ACRA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P02930
UniProtKB:P31224

F

Seeded From UniProt

complete

ECOLI:ACRA

part_of

GO:1990281: efflux pump complex

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P02930
UniProtKB:P31224

C

Seeded From UniProt

complete

ECOLI:ACRB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P0AE06

F

Seeded From UniProt

complete

ECOLI:TOLC

part_of

GO:1990281: efflux pump complex

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P0AE06
UniProtKB:P31224

C

Seeded From UniProt

complete

ECOLI:ACRB

part_of

GO:1990281: efflux pump complex

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P02930
UniProtKB:P0AE06

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.