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PMID:15148392

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Citation

da Silva Xavier, G, Rutter, J and Rutter, GA (2004) Involvement of Per-Arnt-Sim (PAS) kinase in the stimulation of preproinsulin and pancreatic duodenum homeobox 1 gene expression by glucose. Proc. Natl. Acad. Sci. U.S.A. 101:8319-24

Abstract

Per-Arnt-Sim (PAS) domain-containing kinases are common in prokaryotes, but a mammalian counterpart has only recently been described. Although the PAS domain of the mammalian PAS kinase (PASK) is closely related to the bacterial oxygen sensor FixL, it is unclear whether PASK activity is changed in mammalian cells in response to nutrients and might therefore contribute to signal transduction by these or other stimuli. Here, we show that elevated glucose concentrations rapidly increase PASK activity in pancreatic islet beta cells, an event followed by the accumulation of both PASK mRNA and protein. Demonstrating a physiological role for PASK activation, comicroinjection into clonal beta cells of cDNA encoding wild-type PASK, or PASK protein itself, mimics the induction of preproinsulin promoter activity by high glucose concentrations. Conversely, anti-PASK antibodies block promoter activation by the sugar, and the silencing of PASK expression by RNA interference suppresses the up-regulation by glucose of preproinsulin and pancreatic duodenum homeobox 1 gene expression, without affecting glucose-induced changes in the levels of mRNAs encoding glucokinase or uncoupling protein 2. We conclude that PASK is an important metabolic sensor in nutrient-sensitive mammalian cells and plays an unexpected role in the regulation of key genes involved in maintaining the differentiated phenotype of pancreatic beta cells.

Links

PubMed PMC420392 Online version:10.1073/pnas.0307737101

Keywords

Adenosine Triphosphate/metabolism; Animals; Cell Line; Culture Techniques; Gene Expression Regulation; Genes, Reporter; Glucose/metabolism; Homeodomain Proteins; Human Growth Hormone/genetics; Human Growth Hormone/metabolism; Humans; Insulin; Islets of Langerhans/cytology; Islets of Langerhans/metabolism; Proinsulin/genetics; Proinsulin/metabolism; Promoter Regions, Genetic; Protein Precursors/genetics; Protein Precursors/metabolism; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; RNA, Small Interfering/metabolism; Rats; Trans-Activators/genetics; Trans-Activators/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:PDX1

involved_in

GO:0009749: response to glucose

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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