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PMID:15148362

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Citation

Charollais, J, Dreyfus, M and Iost, I (2004) CsdA, a cold-shock RNA helicase from Escherichia coli, is involved in the biogenesis of 50S ribosomal subunit. Nucleic Acids Res. 32:2751-9

Abstract

CsdA, a DEAD-box protein from Escherichia coli, has been proposed to participate in a variety of processes, such as translation initiation, gene regulation after cold-shock, mRNA decay and biogenesis of the small ribosomal subunit. Whether the protein really plays a direct role in these multiple processes is however, not clear. Here, we show that CsdA is involved in the biogenesis of the large rather than the small ribosomal subunit. Deletion of the csdA gene leads to a deficit in free 50S subunits at low temperatures and to the accumulation of a new particle sedimenting around 40S. Analysis of the RNA and protein contents of this particle indicates that it corresponds to a mis-assembled large subunit. Sucrose gradient fractionation shows that in wild-type cells CsdA associates mainly with a pre50S particle. Presumably the RNA helicase activity of CsdA permits a structural rearrangement during 50S biogenesis at low temperature. We showed previously that SrmB, another DEAD-box RNA helicase, is also involved in 50S assembly in E.coli. Our results suggest that CsdA is required at a later step than SrmB. However, over-expression of CsdA corrects the ribosome defect of the srmB-deleted strain, indicating that some functional overlap exists between the two proteins.

Links

PubMed PMC419605 Online version:10.1093/nar/gkh603

Keywords

Cold Temperature; DEAD-box RNA Helicases; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Gene Deletion; Molecular Weight; Phenotype; Polyribosomes/genetics; Polyribosomes/metabolism; Protein Subunits/chemistry; Protein Subunits/deficiency; Protein Subunits/metabolism; RNA Helicases/deficiency; RNA Helicases/genetics; RNA Helicases/metabolism; RNA Precursors/genetics; RNA Precursors/metabolism; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Ribosomal, 23S/genetics; RNA, Ribosomal, 23S/metabolism; Ribosomal Proteins/chemistry; Ribosomal Proteins/metabolism; Ribosomes/chemistry; Ribosomes/genetics; Ribosomes/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:DEAD

acts_upstream_of_or_within

GO:0000027: ribosomal large subunit assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DEAD

involved_in

GO:0000027: ribosomal large subunit assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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