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PMID:15141165

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Citation

Schröder, M, Clark, R, Liu, CY and Kaufman, RJ (2004) The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase. EMBO J. 23:2281-92

Abstract

In Saccharomyces cerevisiae, splicing of HAC1 mRNA is initiated in response to the accumulation of unfolded proteins in the endoplasmic reticulum by the transmembrane kinase-endoribonuclease Ire1p. Spliced Hac1p (Hac1ip) is a negative regulator of differentiation responses to nitrogen starvation, pseudohyphal growth, and meiosis. Here we show that the RPD3-SIN3 histone deacetylase complex (HDAC), its catalytic activity, recruitment of the HDAC to the promoters of early meiotic genes (EMGs) by Ume6p, and the Ume6p DNA-binding site URS1 in the promoters of EMGs are required for nitrogen-mediated negative regulation of EMGs and meiosis by Hac1ip. Co-immunoprecipitation experiments demonstrated that Hac1ip can interact with the HDAC in vivo. Systematic analysis of double deletion strains revealed that HAC1 is a peripheral component of the HDAC. In summary, nitrogen-induced synthesis of Hac1ip and association of Hac1ip with the HDAC are physiological events in the regulation of EMGs by nutrients. These data also define for the first time a gene class that is under negative control by the UPR, and provide the framework for a novel mechanism through which bZIP proteins repress transcription.

Links

PubMed PMC419911 Online version:10.1038/sj.emboj.7600233

Keywords

Binding Sites; Cell Differentiation; DNA-Binding Proteins/metabolism; Fungal Proteins/metabolism; Gene Deletion; Gene Expression Regulation, Fungal; Genes, Fungal; Histone Deacetylases/metabolism; Meiosis; Membrane Glycoproteins/metabolism; Models, Biological; Nitrogen/metabolism; Precipitin Tests; Promoter Regions, Genetic; Protein Folding; Protein-Serine-Threonine Kinases/metabolism; RNA Splicing; Repressor Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Sin3 Histone Deacetylase and Corepressor Complex; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:HAC1

enables

GO:0000981: DNA-binding transcription factor activity, RNA polymerase II-specific

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:HAC1

involved_in

GO:0010674: negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:HAC1

enables

GO:0000981: DNA-binding transcription factor activity, RNA polymerase II-specific

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:HAC1

involved_in

GO:0010674: negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:RPD3

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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