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PMID:15135047

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Citation

Katoh, Y, Fujimoto, M, Nakamura, K, Inouye, S, Sugahara, K, Izu, H and Nakai, A (2004) Hsp25, a member of the Hsp30 family, promotes inclusion formation in response to stress. FEBS Lett. 565:28-32

Abstract

Protein aggregates are oligomeric complexes of misfolded proteins, and serve as the seeds of inclusion bodies termed aggresomes in the cells. Heat shock proteins (Hsps) prevent misfolding and aggregate formation. Here, we found that only avian Hsp25 dominantly accumulated in the aggresomes induced by proteasome inhibition. Molecular cloning of chicken Hsp25 (cHsp25) revealed that it belongs to the Hsp30 family, which is a subfamily of the alpha-crystallin/small Hsp gene family. Unexpectedly, overexpression of cHsp25 into HeLa cells promoted inclusion formation whereas overexpression of mouse Hsp27 and its chicken homologue did not. These results suggest that cHsp25 acts differently from other small Hsps on protein aggregates.

Links

PubMed Online version:10.1016/j.febslet.2003.12.085

Keywords

Amino Acid Sequence; Animals; Avian Proteins/biosynthesis; Blotting, Western; Chickens; Cloning, Molecular; Crystallins/chemistry; DNA, Complementary/metabolism; Fibroblasts/metabolism; HSP27 Heat-Shock Proteins; HSP30 Heat-Shock Proteins; HeLa Cells; Heat-Shock Proteins/biosynthesis; Heat-Shock Proteins/chemistry; Heat-Shock Proteins/physiology; Humans; Inclusion Bodies; Intracellular Signaling Peptides and Proteins; Membrane Proteins/physiology; Microscopy, Fluorescence; Molecular Sequence Data; Neoplasm Proteins/chemistry; Neoplasm Proteins/physiology; Phylogeny; Protein Folding; Protein-Serine-Threonine Kinases/biosynthesis; Quail; Sequence Homology, Amino Acid; Stress, Physiological; Ubiquitin/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:CRYAA

located_in

GO:0005634: nucleus

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

C

Seeded From UniProt

complete

CHICK:CRYAA

located_in

GO:0005737: cytoplasm

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

C

Seeded From UniProt

complete

CHICK:CRYAA

located_in

GO:0070062: extracellular exosome

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

C

Seeded From UniProt

complete

CHICK:HS90A

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:HSPB1

located_in

GO:0005634: nucleus

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

located_in

GO:0005737: cytoplasm

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:HSPB1

located_in

GO:0005622: intracellular anatomical structure

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

located_in

GO:0043292: contractile fiber

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:0032760: positive regulation of tumor necrosis factor production

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:HSPB1

located_in

GO:0030018: Z disc

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

located_in

GO:0005886: plasma membrane

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:Q6BDR9

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:CRYAA

part_of

GO:0070062: extracellular exosome

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

C

Seeded From UniProt

complete

CHICK:CRYAA

involved_in

GO:0051260: protein homooligomerization

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

P

Seeded From UniProt

complete

CHICK:CRYAA

enables

GO:0051082: unfolded protein binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

F

Seeded From UniProt

complete

CHICK:CRYAA

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

P

Seeded From UniProt

complete

CHICK:CRYAA

enables

GO:0042802: identical protein binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

F

Seeded From UniProt

complete

CHICK:CRYAA

involved_in

GO:0032387: negative regulation of intracellular transport

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

P

Seeded From UniProt

complete

CHICK:CRYAA

involved_in

GO:0007601: visual perception

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

P

Seeded From UniProt

complete

CHICK:CRYAA

part_of

GO:0005737: cytoplasm

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

C

Seeded From UniProt

complete

CHICK:CRYAA

part_of

GO:0005634: nucleus

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P02489

C

Seeded From UniProt

complete

CHICK:HS90A

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:HSPB1

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:HSPB1

part_of

GO:0005737: cytoplasm

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

part_of

GO:0005634: nucleus

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

part_of

GO:0005886: plasma membrane

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

part_of

GO:0030018: Z disc

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

part_of

GO:0043292: contractile fiber

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:0042535: positive regulation of tumor necrosis factor biosynthetic process

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:0043122: regulation of I-kappaB kinase/NF-kappaB signaling

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:2001234: negative regulation of apoptotic signaling pathway

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:0032731: positive regulation of interleukin-1 beta production

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:HSPB1

enables

GO:0008426: protein kinase C inhibitor activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

F

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:0006469: negative regulation of protein kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:HSPB1

part_of

GO:0005622: intracellular

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

C

Seeded From UniProt

complete

CHICK:HSPB1

enables

GO:0005080: protein kinase C binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

F

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:0071901: negative regulation of protein serine/threonine kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:HSPB1

involved_in

GO:1902176: negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P14602

P

Seeded From UniProt

complete

CHICK:Q6BDR9

part_of

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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