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PMID:15133499

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Citation

Rehwinkel, J, Herold, A, Gari, K, Köcher, T, Rode, M, Ciccarelli, FL, Wilm, M and Izaurralde, E (2004) Genome-wide analysis of mRNAs regulated by the THO complex in Drosophila melanogaster. Nat. Struct. Mol. Biol. 11:558-66

Abstract

In yeast cells, the THO complex has been implicated in mitotic recombination, transcription elongation and mRNA nuclear export. The stable core of THO consists of Tho2p, Hpr1p, Mft1p and Thp2p. Whether a complex with similar functions assembles in metazoa has not yet been established. Here we report that Drosophila melanogaster THO consists of THO2, HPR1 and three proteins, THOC5-THOC7, which have no orthologs in budding yeast. Gene expression profiling in cells depleted of THO components revealed that <20% of the transcriptome was regulated by THO. Nonetheless, export of heat-shock mRNAs under heat stress was strictly dependent on THO function. Notably, 8% of upregulated genes encode proteins involved in DNA repair. Thus, although THO function seems to be conserved, the vast majority of mRNAs are transcribed and exported independently of THO in D. melanogaster.

Links

PubMed Online version:10.1038/nsmb759

Keywords

Active Transport, Cell Nucleus; Animals; DNA Repair; Drosophila Proteins/physiology; Drosophila melanogaster/genetics; Gene Expression Profiling; Gene Expression Regulation; Genome; Protein Binding; RNA, Messenger/genetics; Transcription Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:E2QCS8

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:E2QCS8

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:M9PE28

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:M9PE28

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:THOC7

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC7

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000305: curator inference used in manual assertion

GO:0000347

P

Seeded From UniProt

complete

DROME:Q960L4

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q960L4

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VNI8

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VNI8

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VQ76

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VQ76

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:THOC6

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC6

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000305: curator inference used in manual assertion

GO:0000347

P

Seeded From UniProt

complete

DROME:THOC5

part_of

GO:0000347: THO complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC5

involved_in

GO:0031990: mRNA export from nucleus in response to heat stress

ECO:0000305: curator inference used in manual assertion

GO:0000347

P

Seeded From UniProt

complete


See also

References

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