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PMID:15110715

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Citation

Galli, LM, Willert, K, Nusse, R, Yablonka-Reuveni, Z, Nohno, T, Denetclaw, W and Burrus, LW (2004) A proliferative role for Wnt-3a in chick somites. Dev. Biol. 269:489-504

Abstract

The proper patterning of somites to give rise to sclerotome, dermomyotome, and myotome involves the coordination of many different cellular processes, including lineage specification, cell proliferation, cell death, and differentiation, by intercellular signals. One such family of secreted signaling proteins known to influence somite patterning is the Wnt family. Although the participation of Wnt-3a in the patterning of dorsal structures in the somite is well established, no clear consensus has emerged about the cellular processes that are governed by Wnt-3a in the somite. The recent demonstration that Wnt-3a has a proliferative role in the neural tube [Development 129 (2002) 2087] suggested that Wnt-3a might also act to regulate proliferation in somites. To test this hypothesis, we first analyzed the effects of Wnt-3a on segmental plate and somite explants (from Hamburger and Hamilton stage 10 chick embryos) grown in culture. These studies indicate that Wnt-3a is capable of maintaining and/or inducing expression of both Pax-3 and Pax-7, transcription factors that have been implicated in proliferation. To directly test for a role in proliferation, explants were immunostained with antibodies against phospho-histone H3. Explants treated with Wnt-3a show an increase in the percentage of cells expressing phospho-histone H3 as compared to controls. To test the proliferative effect of Wnt-3a in vivo, we ectopically expressed Wnt-3a in chick neural tubes via electroporation. Consistent with previous studies, ectopic expression of Wnt-3a in vivo results in a mediolateral expansion of the dermomyotome and myotome. We now show that proliferation of dorsal/dermomyotomal cells is significantly enhanced by ectopic Wnt-3a. Collectively, our explant and in vivo studies indicate that an increase in proliferation plays an important role in the expansion of the dermomyotome and myotome in Wnt-3a-treated embryos. Furthermore, our results demonstrate that small changes in proliferation can dramatically influence patterning and morphogenesis.

Links

PubMed Online version:10.1016/j.ydbio.2004.01.041

Keywords

Animals; Apoptosis; Body Patterning; Cell Division; Chick Embryo; Gene Expression Regulation, Developmental; Muscle Development; Proteins/physiology; Somites/physiology; Wnt Proteins; Wnt3 Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:A0A1D5PX88

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:A0A1D5PX88

located_in

GO:0005640: nuclear outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:A0A1D5PX88

involved_in

GO:0050679: positive regulation of epithelial cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0002329)
  • occurs_in:(UBERON:0004290)
  • occurs_in:(UBERON:0003082)

Seeded From UniProt

complete

CHICK:A5GZA7

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0002329)

Seeded From UniProt

complete

CHICK:WNT3A

located_in

GO:0005640: nuclear outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q8QGS3

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0002329)

Seeded From UniProt

complete

CHICK:Q9PSS2

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0002329)

Seeded From UniProt

complete

CHICK:A5GZA7

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0002329)

Seeded From UniProt

complete

CHICK:WNT3A

part_of

GO:0005640: nuclear outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:WNT3A

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:WNT3A

involved_in

GO:0050679: positive regulation of epithelial cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0002329)
  • occurs_in:(UBERON:0004290)
  • occurs_in:(UBERON:0003082)

Seeded From UniProt

complete

CHICK:Q8QGS3

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0002329)

Seeded From UniProt

complete

CHICK:Q9PSS2

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0002329)

Seeded From UniProt

complete

CHICK:Q9PWH1

part_of

GO:0005640: nuclear outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q9PWH1

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q9PWH1

involved_in

GO:0050679: positive regulation of epithelial cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0002329)
  • occurs_in:(UBERON:0004290)
  • occurs_in:(UBERON:0003082)

Seeded From UniProt

complete

Notes

See also

References

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GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

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Internal error

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[49af0152e46a081381f4350d] /wiki/index.php/PMID:15110715 Error from line 252 of /usr/local/github/wiki-extensions/wiki-extensions-hulab/AnnotationLinks/AnnotationLinks.php: Call to a member function getIdentifier() on null

Backtrace:

#0 /usr/local/github/wiki-extensions/wiki-extensions-hulab/AnnotationLinks/AnnotationLinks.php(70): AnnotationLinks::getQuickGOannotations(string, array)
#1 /var/www/html/go/wiki/includes/parser/Parser.php(3888): AnnotationLinks::renderAnnotations(NULL, array, Parser, PPFrame_DOM)
#2 /var/www/html/go/wiki/includes/parser/Preprocessor_DOM.php(1362): Parser->extensionSubstitution(array, PPFrame_DOM)
#3 /var/www/html/go/wiki/includes/parser/Parser.php(2942): PPFrame_DOM->expand(DOMElement, integer)
#4 /var/www/html/go/wiki/includes/parser/Parser.php(1293): Parser->replaceVariables(string)
#5 /var/www/html/go/wiki/includes/parser/Parser.php(443): Parser->internalParse(string)
#6 /var/www/html/go/wiki/includes/content/WikitextContent.php(323): Parser->parse(string, Title, ParserOptions, boolean, boolean, integer)
#7 /var/www/html/go/wiki/includes/content/AbstractContent.php(516): WikitextContent->fillParserOutput(Title, integer, ParserOptions, boolean, ParserOutput)
#8 /var/www/html/go/wiki/includes/jobqueue/jobs/RefreshLinksJob.php(231): AbstractContent->getParserOutput(Title, integer, ParserOptions, boolean)
#9 /var/www/html/go/wiki/includes/jobqueue/jobs/RefreshLinksJob.php(126): RefreshLinksJob->runForTitle(Title)
#10 /var/www/html/go/wiki/includes/jobqueue/JobRunner.php(296): RefreshLinksJob->run()
#11 /var/www/html/go/wiki/includes/jobqueue/JobRunner.php(193): JobRunner->executeJob(RefreshLinksJob, Wikimedia\Rdbms\LBFactorySimple, BufferingStatsdDataFactory, integer)
#12 /var/www/html/go/wiki/includes/MediaWiki.php(1002): JobRunner->run(array)
#13 /var/www/html/go/wiki/includes/MediaWiki.php(988): MediaWiki->triggerSyncJobs(integer, MediaWiki\Logger\LegacyLogger)
#14 /var/www/html/go/wiki/includes/MediaWiki.php(912): MediaWiki->triggerJobs()
#15 /var/www/html/go/wiki/includes/MediaWiki.php(727): MediaWiki->restInPeace(string, boolean)
#16 /var/www/html/go/wiki/includes/MediaWiki.php(750): MediaWiki->{closure}()
#17 /var/www/html/go/wiki/includes/MediaWiki.php(557): MediaWiki->doPostOutputShutdown(string)
#18 /var/www/html/go/wiki/index.php(42): MediaWiki->run()
#19 {main}

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