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PMID:15110047

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Citation

Nojima, H, Shimizu, T, Kim, CH, Yabe, T, Bae, YK, Muraoka, O, Hirata, T, Chitnis, A, Hirano, T and Hibi, M (2004) Genetic evidence for involvement of maternally derived Wnt canonical signaling in dorsal determination in zebrafish. Mech. Dev. 121:371-86

Abstract

In zebrafish, the program for dorsal specification begins soon after fertilization. Dorsal determinants are localized initially to the vegetal pole, then transported to the blastoderm, where they are thought to activate the canonical Wnt pathway, which induces the expression of dorsal-specific genes. We identified a novel maternal-effect recessive mutation, tokkaebi (tkk), that affects formation of the dorsal axis. Severely ventralized phenotypes, including a lack of dorso-anterior structures, were seen in 5-100% of the embryos obtained from tkk homozygous transmitting females. tkk embryos displayed defects in the nuclear accumulation of beta-catenin on the dorsal side, and reduced or absent expression of dorsal-specific genes. Mesoderm and endoderm formation outside the dorsal axis was not significantly affected. Injection of RNAs for activated beta-catenin, dominant-negative forms of Axin1 and GSK3beta, and wild-type Dvl3, into the tkk embryos suppressed the ventralized phenotypes and/or dorsalized the embryos, and restored or induced an ectopic and expanded expression of bozozok/dharma and goosecoid. However, dorsalization by wnt RNAs was affected in the tkk embryos. Inhibition of cytoplasmic calcium release elicited an ectopic and expanded expression of chordin in the wild-type, but did not restore chordin expression efficiently in the tkk embryos. These data indicate that the tkk gene product functions upstream of or parallel to the beta-catenin-degradation machinery to control the stability of beta-catenin. The tkk locus was mapped to chromosome 16. These data provide genetic evidence that the maternally derived canonical Wnt pathway upstream of beta-catenin is involved in dorsal axis formation in zebrafish.

Links

PubMed Online version:10.1016/j.mod.2004.02.003

Keywords

Animals; Body Patterning/genetics; Body Patterning/physiology; Calcium Signaling/physiology; Cell Nucleus/metabolism; Chromosome Mapping; Crosses, Genetic; Cytoskeletal Proteins/metabolism; Ligands; Organizers, Embryonic/physiology; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; Signal Transduction/genetics; Signal Transduction/physiology; Trans-Activators/metabolism; Wnt Proteins; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins; beta Catenin

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A2R9YJG3

involved_in

GO:2000053: regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete

DANRE:A0A2R9YJG3

involved_in

GO:0060828: regulation of canonical Wnt signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete

DANRE:D3KVM1

involved_in

GO:2000053: regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete

DANRE:D3KVM1

involved_in

GO:0060828: regulation of canonical Wnt signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete

DANRE:D3KVM2

involved_in

GO:2000053: regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete

DANRE:D3KVM2

involved_in

GO:0060828: regulation of canonical Wnt signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete

DANRE:Q08CJ9

involved_in

GO:2000053: regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete

DANRE:Q08CJ9

involved_in

GO:0060828: regulation of canonical Wnt signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-040510-2

P

Seeded From UniProt

complete


See also

References

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