GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15100295

From GONUTS
Jump to: navigation, search
Citation

Yee, CS, Yao, Y, Li, P, Klemsz, MJ, Blum, JS and Chang, CH (2004) Cathepsin E: a novel target for regulation by class II transactivator. J. Immunol. 172:5528-34

Abstract

The aspartic proteinase cathepsin E (CatE) has been implicated in Ag processing. In this study we report that CatE expression is negatively regulated by the MHC class II transactivator (CIITA). CIITA-deficient murine and human B cells expressed greater CatE than wild-type B cells, whereas overexpression of CIITA in a human gastric carcinoma cell line, AGS, resulted in decreased CatE mRNA and protein. AGS cells expressing CIITA also exhibited decreased processing of OVA Ag. Inhibition of CatE expression is specific to the type III CIITA isoform and maps to the acidic and proline/serine/threonine-rich (PST) protein domains of CIITA. We found that CatE expression is inducible by PU.1 and p300, and that this induction can be reversed by CIITA. These findings demonstrate a novel phenomenon: regulation of CatE Ag processing by CIITA in an isoform-dependent manner.

Links

PubMed

Keywords

Animals; Antigen Presentation/genetics; Antigen Presentation/immunology; Cathepsin E/antagonists & inhibitors; Cathepsin E/biosynthesis; Cathepsin E/genetics; Cell Line; Cell Line, Transformed; Cell Line, Tumor; Down-Regulation/genetics; E1A-Associated p300 Protein; Enzyme Precursors/antagonists & inhibitors; Enzyme Precursors/genetics; Enzyme Repression; Genes, Tumor Suppressor; Humans; Isoenzymes/physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; Nuclear Proteins/antagonists & inhibitors; Nuclear Proteins/deficiency; Nuclear Proteins/genetics; Nuclear Proteins/pharmacology; Nuclear Proteins/physiology; Ovalbumin/immunology; Ovalbumin/metabolism; Peptide Fragments/physiology; Proto-Oncogene Proteins/antagonists & inhibitors; Proto-Oncogene Proteins/pharmacology; RNA, Messenger/biosynthesis; Trans-Activators/antagonists & inhibitors; Trans-Activators/deficiency; Trans-Activators/genetics; Trans-Activators/pharmacology; Trans-Activators/physiology; Transfection; Up-Regulation/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CATE

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P70269

F

Seeded From UniProt

complete

MOUSE:C2TA

acts_upstream_of_or_within

GO:0045892: negative regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857456

P

Seeded From UniProt

complete

MOUSE:SPI1

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:EP300

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.