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PMID:15078549

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Citation

Swinny, JD, Metzger, F, IJkema-Paassen, J, Gounko, NV, Gramsbergen, A and van der Want, JJ (2004) Corticotropin-releasing factor and urocortin differentially modulate rat Purkinje cell dendritic outgrowth and differentiation in vitro. Eur. J. Neurosci. 19:1749-58

Abstract

The precise outgrowth and arborization of dendrites is crucial for their function as integrators of signals relayed from axons and, hence, the functioning of the brain. Proper dendritic differentiation is particularly resonant for Purkinje cells as the intrinsic activity of this cell-type is governed by functionally distinct regions of its dendritic tree. Activity-dependent mechanisms, driven by electrical signaling and trophic factors, account for the most active period of dendritogenesis. An as yet unexplored trophic modulator of Purkinje cell dendritic development is corticotropin-releasing factor (CRF) and family member, urocortin, both of which are localized in climbing fibers. Here, we use rat organotypic cerebellar slice cultures to investigate the roles of CRF and urocortin on Purkinje cell dendritic development. Intermittent exposure (12 h per day for 10 days in vitro) of CRF and urocortin induced significantly more dendritic outgrowth (45% and 70%, respectively) and elongation (25% and 15%, respectively) compared with untreated cells. Conversely, constant exposure to CRF and urocortin significantly inhibited dendritic outgrowth. The trophic effects of CRF and urocortin are mediated by the protein kinase A and mitogen-activating protein kinase pathways. The study shows unequivocally that CRF and urocortin are potent regulators of dendritic development. However, their stimulatory or inhibitory effects are dependent upon the degree of expression of these peptides. Furthermore, the effects of CRF and urocortin on neuronal differentiation and re-modeling may provide a cellular basis for pathologies such as major depression, which show perturbations in the expression of these stress peptides.

Links

PubMed Online version:10.1111/j.1460-9568.2004.03279.x

Keywords

Analysis of Variance; Animals; Animals, Newborn; Calcium-Binding Protein, Vitamin D-Dependent/metabolism; Carbazoles/pharmacology; Cell Count/methods; Cell Differentiation/drug effects; Corticotropin-Releasing Hormone/antagonists & inhibitors; Corticotropin-Releasing Hormone/pharmacology; Dendrites/drug effects; Dose-Response Relationship, Drug; Drug Interactions; Enzyme Inhibitors/pharmacology; Flavonoids/pharmacology; Hippocampus/cytology; Humans; Immunohistochemistry/methods; Indoles/pharmacology; Linear Models; Neuroprotective Agents/pharmacology; Purkinje Cells/cytology; Purkinje Cells/drug effects; Pyrimidines/pharmacology; Pyrroles/pharmacology; Rats; Time Factors; Urocortins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:UCN1

involved_in

GO:0031175: neuron projection development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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