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PMID:15044608

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Citation

Bayne, RA, Martins da Silva, SJ and Anderson, RA (2004) Increased expression of the FIGLA transcription factor is associated with primordial follicle formation in the human fetal ovary. Mol. Hum. Reprod. 10:373-81

Abstract

The process of primordial follicle formation is central to the determination of a woman's reproductive lifespan, and in humans occurs towards the end of mid-gestation. Gene knockout analysis in the mouse has shown that Figla, a transcription factor specifically expressed in germ cells, is essential for oocytes to survive and form primordial follicles. Our objective was to investigate whether a human homologue present in the genome database plays a similar role in human ovary development. Standard and real-time RT-PCR demonstrated that the human FIGLA gene is expressed in the fetal ovary but not by a range of other tissues, and that expression increases across mid-gestation, rising some 40-fold by the time of primordial follicle formation. The entire coding sequence was cloned and new exonic sequences identified. Electrophoretic mobility shift assays with in vitro-expressed human FIGLA protein showed that, as in the mouse, FIGLA can heterodimerize with E12 protein and bind to the E-box of the human ZP2 promoter. Similar mobility shifts were identified in human fetal ovary extracts. These results suggest that FIGLA is involved in continued oocyte survival as primordial follicles form in the human as in the rodent ovary.

Links

PubMed Online version:10.1093/molehr/gah056

Keywords

Amino Acid Sequence; Animals; Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Dimerization; Female; Gene Expression Regulation, Developmental; Helix-Loop-Helix Motifs; Humans; Male; Mice; Molecular Sequence Data; Ovarian Follicle/cytology; Ovarian Follicle/growth & development; Ovarian Follicle/physiology; Ovary/anatomy & histology; Ovary/growth & development; Pregnancy; Promoter Regions, Genetic; Protein Binding; TCF Transcription Factors; Testis/chemistry; Testis/metabolism; Tissue Extracts; Transcription Factor 7-Like 1 Protein; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:FIGLA

part_of

GO:0005667: transcription regulator complex

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q6QHK4

C

Seeded From UniProt

complete

MOUSE:FIGLA

enables

GO:0043425: bHLH transcription factor binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:Q6QHK4

F

Seeded From UniProt

complete

HUMAN:TFE2

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:TFE2

enables

GO:0140297: DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q6QHK4

F

Seeded From UniProt

complete

HUMAN:TFE2

contributes_to

GO:0070888: E-box binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q6QHK4

F

Seeded From UniProt

complete

HUMAN:FIGLA

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:FIGLA

enables

GO:0043425: bHLH transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P15923-1

F

Seeded From UniProt

complete

HUMAN:FIGLA

involved_in

GO:0048599: oocyte development

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:FIGLA

contributes_to

GO:0070888: E-box binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P15923-1

F

Seeded From UniProt

complete

HUMAN:TFE2

enables

GO:0008134: transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q6QHK4

F

Seeded From UniProt

complete

HUMAN:TFE2

part_of

GO:0005667: transcription factor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:TFE2

contributes_to

GO:0043565: sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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