GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:15035984
| Citation |
Cook, HA, Koppetsch, BS, Wu, J and Theurkauf, WE (2004) The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification. Cell 116:817-29 |
|---|---|
| Abstract |
Polarization of the microtubule cytoskeleton during early oogenesis is required to specify the posterior of the Drosophila oocyte, which is essential for asymmetric mRNA localization during mid-oogenesis and for embryonic axis specification. The posterior determinant oskar mRNA is translationally silent until mid-oogenesis. We show that mutations in armitage and three components of the RNAi pathway disrupt oskar mRNA translational silencing, polarization of the microtubule cytoskeleton, and posterior localization of oskar mRNA. armitage encodes a homolog of SDE3, a presumptive RNA helicase involved in posttranscriptional gene silencing (RNAi) in Arabidopsis, and is required for RNAi in Drosophila ovaries. Armitage forms an asymmetric network associated with the polarized microtubule cytoskeleton and is concentrated with translationally silent oskar mRNA in the oocyte. We conclude that RNA silencing is essential for establishment of the cytoskeletal polarity that initiates embryonic axis specification and for translational control of oskar mRNA. |
| Links | |
| Keywords |
Amino Acid Sequence/genetics; Animals; Arabidopsis Proteins/genetics; Base Sequence/genetics; Body Patterning/genetics; Cell Polarity/genetics; Cytoskeleton/genetics; Cytoskeleton/metabolism; DNA, Complementary/analysis; DNA, Complementary/genetics; Drosophila Proteins/genetics; Drosophila Proteins/isolation & purification; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/embryology; Embryo, Nonmammalian/metabolism; Female; Microtubules/genetics; Microtubules/metabolism; Molecular Sequence Data; Mutation; Oocytes/cytology; Oocytes/metabolism; Protein Biosynthesis/genetics; RNA Helicases/genetics; RNA Helicases/isolation & purification; RNA Interference/physiology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
