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PMID:15014435

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Citation

Girard, KD, Chaney, C, Delannoy, M, Kuo, SC and Robinson, DN (2004) Dynacortin contributes to cortical viscoelasticity and helps define the shape changes of cytokinesis. EMBO J. 23:1536-46

Abstract

During cytokinesis, global and equatorial pathways deform the cell cortex in a stereotypical manner, which leads to daughter cell separation. Equatorial forces are largely generated by myosin-II and the actin crosslinker, cortexillin-I. In contrast, global mechanics are determined by the cortical cytoskeleton, including the actin crosslinker, dynacortin. We used direct morphometric characterization and laser-tracking microrheology to quantify cortical mechanical properties of wild-type and cortexillin-I and dynacortin mutant Dictyostelium cells. Both cortexillin-I and dynacortin influence cytokinesis and interphase cortical viscoelasticity as predicted from genetics and biochemical data using purified dynacortin proteins. Our studies suggest that the regulation of cytokinesis ultimately requires modulation of proteins that control the cortical mechanical properties that establish the force-balance that specifies the shapes of cytokinesis. The combination of genetic, biochemical, and biophysical observations suggests that the cell's cortical mechanical properties control how the cortex is remodeled during cytokinesis.

Links

PubMed PMC391072 Online version:10.1038/sj.emboj.7600167

Keywords

Actins/metabolism; Animals; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Shape; Cytokinesis/physiology; Cytoplasm/metabolism; Cytoskeleton/metabolism; Cytoskeleton/ultrastructure; Dictyostelium/cytology; Dictyostelium/metabolism; Elasticity; Lasers; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Protozoan Proteins; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Rheology/methods

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:CTXA

part_of

GO:0005826: actomyosin contractile ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:CTXA

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q54HG2

F

Seeded From UniProt

complete

DICDI:Q9NHF6

acts_upstream_of_or_within

GO:0000281: mitotic cytokinesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q9NHF6

colocalizes_with

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:Q9NHF6

involved_in

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q9NHF6

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9NHF6

F

Seeded From UniProt

complete

DICDI:Q9NHF6

part_of

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DICDI:Q9NHF6

enables

GO:0051015: actin filament binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DICDI:Q9NHF6

acts_upstream_of_or_within

GO:1903664: regulation of asexual reproduction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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