GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15004221

From GONUTS
Jump to: navigation, search
Citation

Bertling, E, Hotulainen, P, Mattila, PK, Matilainen, T, Salminen, M and Lappalainen, P (2004) Cyclase-associated protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol. Biol. Cell 15:2324-34

Abstract

Cyclase-associated proteins (CAPs) are highly conserved actin monomer binding proteins present in all eukaryotes. However, the mechanism by which CAPs contribute to actin dynamics has been elusive. In mammals, the situation is further complicated by the presence of two CAP isoforms whose differences have not been characterized. Here, we show that CAP1 is widely expressed in mouse nonmuscle cells, whereas CAP2 is the predominant isoform in developing striated muscles. In cultured NIH3T3 and B16F1 cells, CAP1 is a highly abundant protein that colocalizes with cofilin-1 to dynamic regions of the cortical actin cytoskeleton. Analysis of CAP1 knockdown cells demonstrated that this protein promotes rapid actin filament depolymerization and is important for cell morphology, migration, and endocytosis. Interestingly, depletion of CAP1 leads to an accumulation of cofilin-1 into abnormal cytoplasmic aggregates and to similar cytoskeletal defects to those seen in cofilin-1 knockdown cells, demonstrating that CAP1 is required for proper subcellular localization and function of ADF/cofilin. Together, these data provide the first direct in vivo evidence that CAP promotes rapid actin dynamics in conjunction with ADF/cofilin and is required for several central cellular processes in mammals.

Links

PubMed PMC404026 Online version:10.1091/mbc.E04-01-0048

Keywords

Actin Cytoskeleton/metabolism; Actin Depolymerizing Factors; Actins/analysis; Actins/metabolism; Adaptor Proteins, Signal Transducing; Animals; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Cycle Proteins/physiology; Cell Line; Cell Movement/physiology; Cytoskeletal Proteins/analysis; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Cytoskeletal Proteins/physiology; Destrin; Endocytosis/physiology; Gene Expression; Gene Silencing; In Situ Hybridization; Mice; Microfilament Proteins/analysis; Microfilament Proteins/metabolism; Muscle, Skeletal/cytology; Muscle, Skeletal/metabolism; Protein Isoforms/metabolism; RNA Interference; RNA, Messenger/analysis; Saccharomyces cerevisiae Proteins/analysis; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:COF1

located_in

GO:0030864: cortical actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CAP1

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CAP1

acts_upstream_of_or_within

GO:0001667: ameboidal-type cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CAP1

located_in

GO:0030864: cortical actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CAP1

acts_upstream_of_or_within

GO:0006898: receptor-mediated endocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:COF1

part_of

GO:0030864: cortical actin cytoskeleton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.