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PMID:1496003

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Citation

Prakash, K, Fang, XD, Engelberg, D, Behal, A and Parker, CS (1992) dOct2, a Drosophila Oct transcription factor that functions in yeast. Proc. Natl. Acad. Sci. U.S.A. 89:7080-4

Abstract

Oct factors are members of the POU family of transcription factors that are shown to play important roles during development in mammals. Here we report the cDNA cloning and expression of a Drosophila Oct transcription factor. Whole mount in situ hybridization experiments revealed that the spatial expression patterns of this gene during embryonic development have not yet been observed for any other gene. In early embryogenesis, its transcripts are transiently expressed as a wide uniform band from 20% to 40% of the egg length, very similar to that of gap genes. This pattern progressively resolves into a series of narrower stripes followed by expression in 14 stripes. Subsequently, transcripts from this gene are expressed in the central nervous system and the brain. When expressed in the yeast Saccharomyces cerevisiae, this Drosophila factor functions as a strong, octamer-dependent activator of transcription. Our data strongly suggest possible functions for the Oct factor in pattern formation in Drosophila that might transcend the boundaries of genetically defined segmentation genes.

Links

PubMed PMC49649

Keywords

Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA/genetics; DNA-Binding Proteins/metabolism; Drosophila/genetics; Drosophila/metabolism; Embryo, Nonmammalian; Gene Library; Humans; Molecular Sequence Data; Octamer Transcription Factor-2; Plasmids; Protein Conformation; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/genetics; Sequence Homology, Nucleic Acid; Transcription Factors/genetics; Transcription Factors/isolation & purification; Transcription Factors/metabolism; beta-Galactosidase/genetics; beta-Galactosidase/isolation & purification; beta-Galactosidase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:PDM2A

located_in

GO:0005634: nucleus

ECO:0000305: curator inference used in manual assertion

GO:0006355

C

Seeded From UniProt

complete

DROME:PDM2A

involved_in

GO:0007417: central nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PDM2A

involved_in

GO:0007398: ectoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PDM2A

involved_in

GO:0006355: regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PDM2A

enables

GO:0003677: DNA binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:PDM2B

located_in

GO:0005634: nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:PDM2B

involved_in

GO:0007417: central nervous system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PDM2B

involved_in

GO:0007398: ectoderm development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PDM2B

involved_in

GO:0006355: regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PDM2B

enables

GO:0003677: DNA binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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