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PMID:14769882

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Citation

Nagita, M, Inoue, H, Nakamura, N and Kanazawa, H (2003) Two nuclear export signals specify the cytoplasmic localization of calcineurin B homologous protein 1. J. Biochem. 134:919-25

Abstract

We previously showed that calcineurin B homologous protein 1 (CHP1) interacts with nuclear apoptosis-inducing protein kinase DRAK2, and that overexpression of DRAK2 induces the nuclear accumulation of CHP1, although CHP1 usually resides in the cytoplasm [Matsumoto et al. (2001) J. Biochem. 130, 217-225]. Here we show that CHP1 has two functional nuclear export signal (NES) sequences in its carboxyl-terminal region. Treatment of several cell lines with leptomycin B, a specific inhibitor of CRM1-dependent nuclear export, induces the nuclear accumulation of CHP1. Moreover, CHP1-GFP fusion proteins with deletions or point mutations affecting the two putative NES sequences accumulate in the nucleus to a greater extent than wild-type CHP1-GFP. Tagging glutathione S-transferase-GFP fusion protein with each NES sequence caused a shift in their intracellular localization from all over the cells to the cytoplasm. These results suggest that after CHP1 has entered the nucleus, it is exported to the cytoplasm in an NES-dependent manner.

Links

PubMed

Keywords

Active Transport, Cell Nucleus/genetics; Amino Acid Sequence; Animals; CHO Cells; COS Cells; Calcineurin/chemistry; Calcineurin/genetics; Calcineurin/metabolism; Calcium-Binding Proteins/analysis; Calcium-Binding Proteins/biosynthesis; Calcium-Binding Proteins/genetics; Cell Nucleus/chemistry; Cell Nucleus/genetics; Cell Nucleus/metabolism; Cercopithecus aethiops; Cricetinae; Cytoplasm/chemistry; Cytoplasm/genetics; Cytoplasm/metabolism; Green Fluorescent Proteins; Humans; Intracellular Fluid/chemistry; Intracellular Fluid/metabolism; Luminescent Proteins/genetics; Mice; Molecular Sequence Data; NIH 3T3 Cells; Protein Isoforms/chemistry; Protein Isoforms/genetics; Protein Isoforms/metabolism; Rats

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:CHP1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CHP1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CHP1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CHP1

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CHP1

involved_in

GO:0006611: protein export from nucleus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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