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PMID:14726532

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Citation

Walker, AK, Shi, Y and Blackwell, TK (2004) An extensive requirement for transcription factor IID-specific TAF-1 in Caenorhabditis elegans embryonic transcription. J. Biol. Chem. 279:15339-47

Abstract

The general transcription factor TFIID sets the mRNA start site and consists of TATA-binding protein and associated factors (TAF(II)s), some of which are also present in SPT-ADA-GCN5 (SAGA)-related complexes. In yeast, results of multiple studies indicate that TFIID-specific TAF(II)s are not required for the transcription of most genes, implying that intact TFIID may have a surprisingly specialized role in transcription. Relatively little is known about how TAF(II)s contribute to metazoan transcription in vivo, especially at developmental and tissue-specific genes. Previously, we investigated functions of four shared TFIID/SAGA TAF(II)s in Caenorhabditis elegans. Whereas TAF-4 was required for essentially all embryonic transcription, TAF-5, TAF-9, and TAF-10 were dispensable at multiple developmental and other metazoan-specific promoters. Here we show evidence that in C. elegans embryos transcription of most genes requires TFIID-specific TAF-1. TAF-1 is not as universally required as TAF-4, but it is essential for a greater proportion of transcription than TAF-5, -9, or -10 and is important for transcription of many developmental and other metazoan-specific genes. TAF-2, which binds core promoters with TAF-1, appears to be required for a similarly substantial proportion of transcription. C. elegans TAF-1 overlaps functionally with the coactivator p300/CBP (CBP-1), and at some genes it is required along with the TBP-like protein TLF(TRF2). We conclude that during C. elegans embryogenesis TAF-1 and TFIID have broad roles in transcription and development and that TFIID and TLF may act together at certain promoters. Our findings imply that in metazoans TFIID may be of widespread importance for transcription and for expression of tissue-specific genes.

Links

PubMed Online version:10.1074/jbc.M310731200

Keywords

Animals; Caenorhabditis elegans/embryology; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/physiology; Chromosomal Proteins, Non-Histone/metabolism; Chromosomal Proteins, Non-Histone/physiology; Gene Expression Regulation, Developmental; Green Fluorescent Proteins; Histone Chaperones; Humans; Immunoblotting; Luminescent Proteins/metabolism; Microscopy, Fluorescence; Models, Biological; Models, Genetic; Nuclear Proteins/metabolism; Phenotype; Phosphorylation; Phylogeny; Promoter Regions, Genetic; Protein Structure, Tertiary; RNA Interference; Recombinant Fusion Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; TATA Box Binding Protein-Like Proteins/physiology; TATA-Binding Protein Associated Factors/metabolism; Trans-Activators/metabolism; Transcription Factor TFIID/chemistry; Transcription Factor TFIID/metabolism; Transcription Factors/metabolism; Transcription Factors/physiology; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:TBPL1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:TBPL1

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:G5EF68

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:TAF1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:TAF1

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:TAF1

involved_in

GO:0006367: transcription initiation from RNA polymerase II promoter

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:TAF1

involved_in

GO:0006367: transcription initiation from RNA polymerase II promoter

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00000366

P

Seeded From UniProt

complete

CAEEL:TAF1

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P21675

F

Seeded From UniProt

complete

CAEEL:TAF1

contributes_to

GO:0000976: transcription cis-regulatory region binding

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P21675

F

Seeded From UniProt

complete

CAEEL:TAF1

enables

GO:0004402: histone acetyltransferase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P21675

F

Seeded From UniProt

complete

CAEEL:TAF1

part_of

GO:0005669: transcription factor TFIID complex

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:P21675

C

Seeded From UniProt

complete

CAEEL:O61707

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:RPB1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:RPB1

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:CBP1

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00006382

P

Seeded From UniProt

complete

CAEEL:Q21172

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q95ZS0

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:TAF2

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:TAF2

involved_in

GO:0006367: transcription initiation from RNA polymerase II promoter

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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