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PMID:14723709

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Citation

Maeda, T, Sugiura, R, Kita, A, Saito, M, Deng, L, He, Y, Yabin, L, Fujita, Y, Takegawa, K, Shuntoh, H and Kuno, T (2004) Pmr1, a P-type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast: the importance of Mn2+ homeostasis. Genes Cells 9:71-82

Abstract

Schizosaccharomyces pombe pmr1+ gene is homologous to Saccharomyces cerevisiae PMR1 gene, which encodes the P-type Ca2+/Mn2+-ATPase. Addition of Mn2+, as well as Ca2+, to the medium induced pmr1+ gene expression in a calcineurin-dependent manner. The pmr1 knockout (Deltapmr1) cells exhibited hypersensitivity to EGTA. A screen for high gene dosage-suppressors of the EGTA-hypersensitive phenotype of Deltapmr1 led to the identification of pdt1+ gene, which encodes an Nramp-related metal transporter. The Deltapmr1 cells showed round cell morphology. Although Deltapdt1 cells appeared normal in the regular medium, it showed round cell morphology similar to that of the Deltapmr1 cells when Mn2+ was removed from the medium. The removal of Mn2+ also exacerbated the round morphology of the Deltapmr1 cells. The Deltapmr1Deltapdt1 double mutants grew very slowly and showed extremely aberrant cell morphology with round, enlarged and depolarized shape. The addition of Mn2+, but not Ca2+, to the medium completely suppressed the morphological defects, while both Mn2+ and Ca2+ markedly improved the slow growth of the double mutants. These results suggest that Pmr1 and Pdt1 cooperatively regulate cell morphogenesis through the control of Mn2+ homeostasis, and that calcineurin functions as a Mn2+ sensor as well as a Mn2+ homeostasis regulator.

Links

PubMed

Keywords

Calcineurin/metabolism; Calcium/metabolism; Calcium-Transporting ATPases/drug effects; Calcium-Transporting ATPases/genetics; Calcium-Transporting ATPases/metabolism; Cathepsin A/metabolism; Cation Transport Proteins/genetics; Chelating Agents/pharmacology; Egtazic Acid/pharmacology; Manganese/metabolism; Molecular Chaperones/drug effects; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Morphogenesis/physiology; Mutation; RNA, Messenger/metabolism; Saccharomyces cerevisiae Proteins/drug effects; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Schizosaccharomyces/genetics; Schizosaccharomyces/growth & development; Schizosaccharomyces/metabolism; Schizosaccharomyces pombe Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:ATC1

enables

GO:0015410: ABC-type manganese transporter activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

SCHPO:ATC1

involved_in

GO:0070588: calcium ion transmembrane transport

ECO:0000316: genetic interaction evidence used in manual assertion

PomBase:SPAC27F1.08

P

Seeded From UniProt

complete

SCHPO:ATC1

involved_in

GO:0071421: manganese ion transmembrane transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:PDT1

involved_in

GO:0030026: cellular manganese ion homeostasis

ECO:0000316: genetic interaction evidence used in manual assertion

PomBase:SPBC31E1.02c

P

Seeded From UniProt

complete

SCHPO:PDT1

GO:0071421: manganese ion transmembrane transport

ECO:0000315:

P

complete

SCHPO:PDT1

involved_in

GO:0071421: manganese ion transmembrane transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:PDT1

GO:0005384: manganese ion transmembrane transporter activity

ECO:0000304:

F

complete

SCHPO:PDT1

enables

GO:0005384: manganese ion transmembrane transporter activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

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