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PMID:14718386

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Citation

Lombardo, F, Komatsu, D and Hadjiargyrou, M (2004) Molecular cloning and characterization of Mustang, a novel nuclear protein expressed during skeletal development and regeneration. FASEB J. 18:52-61

Abstract

Bone regeneration occurs as a series of events that requires temporal and spatial orchestration of numerous cell types guided by the transcriptional activity of thousands of genes, as recently demonstrated by our laboratory. Using the rat femoral fracture model, bioinformatics, cloning, expression assays, fusion proteins, and transfection, we report on the identification and characterization of one such differentially expressed gene, termed Mustang (musculoskeletal temporally activated novel gene). Mustang encodes for an 82 amino acid nuclear protein with no homology to any known protein family. However, other species homologues (mouse, human, cow) were identified within EST (expressed sequence tag) databases. Nuclear localization was confirmed using a GFP-Mustang fusion protein. Using in situ hybridization, Mustang expression was localized to differentiating periosteal osteogenic cells, proliferating chondrocytes, and osteoblasts of the fracture callus. Unlike adult tissues, developing embryos abundantly express Mustang, especially in mesenchymal condensations of limbs, vertebral perichondrium, and mesenchymal cells of the intervertebral discs. Although the precise function of Mustang is unknown, its unique pattern of expression during bone development and regeneration, absence in adult tissues (except skeletal muscle and tendon), and nuclear localization suggest that Mustang is involved in the development and regeneration of the mammalian musculoskeletal system.

Links

PubMed Online version:10.1096/fj.03-0521com

Keywords

Amino Acid Sequence; Animals; Base Sequence; Bone Development; Bone and Bones/embryology; Bone and Bones/metabolism; Cell Line; Cell Nucleus/chemistry; Cloning, Molecular; Fracture Healing; Gene Expression; Male; Molecular Sequence Data; Nuclear Proteins/analysis; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; RNA, Messenger/metabolism; Rats; Rats, Sprague-Dawley; Recombinant Fusion Proteins/analysis; Recombinant Fusion Proteins/biosynthesis; Sequence Alignment; Tissue Distribution

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:MSTN1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:MSTN1

involved_in

GO:0030326: embryonic limb morphogenesis

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:MSTN1

located_in

GO:0005654: nucleoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:MSTN1

involved_in

GO:0042246: tissue regeneration

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:MSTN1

involved_in

GO:0042060: wound healing

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:MSTN1

located_in

GO:0005634: nucleus

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:Q80XX4

C

Seeded From UniProt

complete


See also

References

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